Detailed information of TA system TA150405

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3689887..3690581
• Replicon: chromosome
• Accession: NZ_CP049081
• Organism: Escherichia coli strain p10A

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q47157
• Locus tag: G6P90_RS18010
• Protein ID: WP_001263489.1
• Coordinates: 3689887..3690285 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G6P90_RS18015
• Protein ID: WP_000554758.1
• Coordinates: 3690288..3690581 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3685475..3685555	-	81	NuclAT_10	-	-
-	3685475..3685555	-	81	NuclAT_10	-	-
-	3685475..3685555	-	81	NuclAT_10	-	-
-	3685475..3685555	-	81	NuclAT_10	-	-
G6P90_RS17985	3686151..3686609	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G6P90_RS17990	3686870..3688327	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
G6P90_RS17995	3688384..3688905	-	522	Protein_3512	peptide chain release factor H	-
G6P90_RS18000	3688901..3689107	-	207	Protein_3513	RtcB family protein	-
G6P90_RS18005	3689425..3689877	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
G6P90_RS18010	3689887..3690285	-	399	WP_001263489.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G6P90_RS18015	3690288..3690581	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G6P90_RS18020	3690633..3691688	-	1056	WP_001226164.1	DNA polymerase IV	-
G6P90_RS18025	3691759..3692544	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
G6P90_RS18030	3692489..3694228	+	1740	WP_001334802.1	flagellar biosynthesis protein FlhA	-
G6P90_RS18035	3694452..3694949	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15487.84 Da
• Isoelectric Point: 7.4215

>T150405 WP_001263489.1 NZ_CP049081:c3690285-3689887 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150405 NZ_CP064680:c3227812-3227498 [Escherichia coli K-12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT150405 WP_000554758.1 NZ_CP049081:c3690581-3690288 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150405 NZ_CP064680:c3227501-3227085 [Escherichia coli K-12]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: A0A090J8B1

Antitoxin

• AlphaFold DB: S1QAE3