Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 631247..631974 Replicon chromosome
Accession NZ_CP049081
Organism Escherichia coli strain p10A

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag G6P90_RS03090 Protein ID WP_000550189.1
Coordinates 631247..631561 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G6P90_RS03095 Protein ID WP_000560266.1
Coordinates 631558..631974 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6P90_RS03070 627414..628400 - 987 WP_000617698.1 Gfo/Idh/MocA family oxidoreductase -
G6P90_RS03075 628479..629162 - 684 WP_001183043.1 vancomycin high temperature exclusion protein -
G6P90_RS03080 629239..629742 - 504 WP_001295542.1 M48 family metallopeptidase -
G6P90_RS03085 629827..630963 + 1137 WP_000018681.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
G6P90_RS03090 631247..631561 + 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
G6P90_RS03095 631558..631974 + 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
G6P90_RS03100 632019..634037 - 2019 WP_000121433.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
G6P90_RS03105 634463..636814 - 2352 WP_000695487.1 alpha-glucosidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T150389 WP_000550189.1 NZ_CP049081:631247-631561 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T150389 NZ_CP064680:c1264731-1264624 [Escherichia coli K-12]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT150389 WP_000560266.1 NZ_CP049081:631558-631974 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT150389 NZ_CP064680:1264779-1264845 [Escherichia coli K-12]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References