Detailed information of TA system TA150378

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 19926..20159
• Replicon: plasmid p11A_p2
• Accession: NZ_CP049079
• Organism: Escherichia coli strain p11A

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: G6P89_RS26555
• Protein ID: WP_001312861.1
• Coordinates: 20001..20159 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19926..19957 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P89_RS26520	16047..16544	+	498	WP_042039995.1	single-stranded DNA-binding protein	-
G6P89_RS26525	16612..16845	+	234	WP_000005990.1	DUF905 family protein	-
G6P89_RS26530	16873..17070	+	198	Protein_21	hypothetical protein	-
G6P89_RS26535	17125..17559	+	435	WP_000845937.1	conjugation system SOS inhibitor PsiB	-
G6P89_RS26540	17556..18275	+	720	WP_001276238.1	plasmid SOS inhibition protein A	-
G6P89_RS26545	18287..18475	-	189	WP_001299721.1	hypothetical protein	-
-	18287..18484	+	198	NuclAT_0	-	-
-	18287..18484	+	198	NuclAT_0	-	-
-	18287..18484	+	198	NuclAT_0	-	-
-	18287..18484	+	198	NuclAT_0	-	-
G6P89_RS26550	18532..19901	+	1370	WP_085947770.1	IS3-like element IS150 family transposase	-
-	19926..19957	+	32	NuclAT_1	-	Antitoxin
-	19926..19957	+	32	NuclAT_1	-	Antitoxin
-	19926..19957	+	32	NuclAT_1	-	Antitoxin
-	19926..19957	+	32	NuclAT_1	-	Antitoxin
G6P89_RS26555	20001..20159	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
G6P89_RS26560	21080..21367	+	288	WP_000107535.1	hypothetical protein	-
G6P89_RS26565	21485..22306	+	822	WP_001234426.1	DUF945 domain-containing protein	-
G6P89_RS26570	22603..23205	-	603	WP_013362798.1	transglycosylase SLT domain-containing protein	-
G6P89_RS26575	23526..23909	+	384	WP_001151524.1	relaxosome protein TraM	-
G6P89_RS26580	24096..24785	+	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	tet(A) / mph(A) / sul1 / qacE / aadA5 / aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15	senB	1..180962	180962

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T150378 WP_001312861.1 NZ_CP049079:20001-20159 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T150378 NZ_CP064679:c3227814-3227500 [Escherichia coli K-12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Nucleotide

• Length: 32 bp

>AT150378 NZ_CP049079:19926-19957 [Escherichia coli]
CACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin