Detailed information of TA system TA150376

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 5084836..5085438
• Replicon: chromosome
• Accession: NZ_CP049077
• Organism: Escherichia coli strain p11A

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: G6P89_RS24950
• Protein ID: WP_000897302.1
• Coordinates: 5085127..5085438 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G6P89_RS24945
• Protein ID: WP_000356397.1
• Coordinates: 5084836..5085126 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P89_RS24915	5080143..5081072	+	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-
G6P89_RS24920	5081254..5081496	-	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
G6P89_RS24925	5081786..5082634	+	849	WP_001038650.1	hypothetical protein	-
G6P89_RS24930	5082950..5083399	+	450	WP_032140890.1	hypothetical protein	-
G6P89_RS24935	5083584..5083802	-	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
G6P89_RS24940	5084199..5084477	-	279	WP_001296612.1	hypothetical protein	-
G6P89_RS24945	5084836..5085126	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G6P89_RS24950	5085127..5085438	-	312	WP_000897302.1	hypothetical protein	Toxin
G6P89_RS24955	5085667..5086575	+	909	WP_001385591.1	alpha/beta hydrolase	-
G6P89_RS24960	5086639..5087580	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G6P89_RS24965	5087625..5088062	-	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G6P89_RS24970	5088059..5088931	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G6P89_RS24975	5088925..5089524	-	600	WP_001296610.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T150376 WP_000897302.1 NZ_CP049077:c5085438-5085127 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150376 NZ_CP064679:c3034182-3033775 [Escherichia coli K-12]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT150376 WP_000356397.1 NZ_CP049077:c5085126-5084836 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150376 NZ_CP064679:c3034429-3034163 [Escherichia coli K-12]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1P416