Detailed information of TA system TA150355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1934049..1934551
• Replicon: chromosome
• Accession: NZ_CP049077
• Organism: Escherichia coli strain p11A

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: E2QNP2
• Locus tag: G6P89_RS09115
• Protein ID: WP_000767819.1
• Coordinates: 1934297..1934551 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: E2QNP3
• Locus tag: G6P89_RS09110
• Protein ID: WP_001259253.1
• Coordinates: 1934049..1934300 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G6P89_RS09085	1929227..1930294	-	1068	WP_000080057.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-
G6P89_RS09090	1930294..1931364	-	1071	WP_000108967.1	histidinol-phosphate transaminase	-
G6P89_RS09095	1931361..1932665	-	1305	WP_001296211.1	histidinol dehydrogenase	-
G6P89_RS09100	1932671..1933570	-	900	WP_000131782.1	ATP phosphoribosyltransferase	-
G6P89_RS09105	1933716..1933766	-	51	WP_001364200.1	his operon leader peptide	-
G6P89_RS09110	1934049..1934300	+	252	WP_001259253.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
G6P89_RS09115	1934297..1934551	+	255	WP_000767819.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
G6P89_RS09120	1934634..1935458	+	825	WP_000754737.1	SDR family oxidoreductase	-
G6P89_RS09125	1935504..1936433	+	930	WP_000803366.1	LysR family transcriptional regulator	-
G6P89_RS09130	1936648..1936710	+	63	WP_010723108.1	membrane protein YoeI	-
G6P89_RS09135	1936700..1938058	+	1359	WP_000019194.1	putrescine/proton symporter PlaP	-
G6P89_RS09140	1938275..1938733	+	459	WP_001531805.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1938275..1938733	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10110.53 Da
• Isoelectric Point: 7.2749

>T150355 WP_000767819.1 NZ_CP049077:1934297-1934551 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T150355 NZ_CP064678:4438510-4438860 [Escherichia coli K-12]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9365.62 Da
• Isoelectric Point: 4.6127

>AT150355 WP_001259253.1 NZ_CP049077:1934049-1934300 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT150355 NZ_CP064678:4438265-4438516 [Escherichia coli K-12]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: E2QNP2

Antitoxin

• AlphaFold DB: E2QNP3