Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 600192..600991 Replicon chromosome
Accession NZ_CP049077
Organism Escherichia coli strain p11A

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag G6P89_RS02915 Protein ID WP_000347251.1
Coordinates 600192..600656 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag G6P89_RS02920 Protein ID WP_001296435.1
Coordinates 600656..600991 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G6P89_RS02885 595193..595627 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G6P89_RS02890 595645..596523 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G6P89_RS02895 596513..597292 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G6P89_RS02900 597303..597776 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G6P89_RS02905 597799..599079 - 1281 WP_000681950.1 tagatose-bisphosphate aldolase subunit KbaZ -
G6P89_RS02910 599328..600137 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G6P89_RS02915 600192..600656 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G6P89_RS02920 600656..600991 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G6P89_RS02925 601140..602711 - 1572 WP_001273763.1 galactarate dehydratase -
G6P89_RS02930 603086..604420 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
G6P89_RS02935 604436..605206 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T150351 WP_000347251.1 NZ_CP049077:c600656-600192 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T150351 NZ_CP064678:c3227814-3227500 [Escherichia coli K-12]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT150351 WP_001296435.1 NZ_CP049077:c600991-600656 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150351 NZ_CP064678:c3227503-3227087 [Escherichia coli K-12]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References