Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3383606..3384235 Replicon chromosome
Accession NZ_CP049055
Organism Candidatus Kuenenia stuttgartiensis strain CSTR1

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag KsCSTR_RS15030 Protein ID WP_099326265.1
Coordinates 3383942..3384235 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag KsCSTR_RS15025 Protein ID WP_099326266.1
Coordinates 3383606..3383923 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KsCSTR_RS15010 3380589..3382114 - 1526 Protein_2971 IS1380-like element ISCku9 family transposase -
KsCSTR_RS15015 3382298..3382855 - 558 WP_157820667.1 GNAT family N-acetyltransferase -
KsCSTR_RS15020 3383059..3383439 - 381 WP_099326267.1 hypothetical protein -
KsCSTR_RS15025 3383606..3383923 - 318 WP_099326266.1 HigA family addiction module antidote protein Antitoxin
KsCSTR_RS15030 3383942..3384235 - 294 WP_099326265.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
KsCSTR_RS19765 3384840..3384956 - 117 WP_197705232.1 hypothetical protein -
KsCSTR_RS15040 3385254..3385382 - 129 WP_197705231.1 type II toxin-antitoxin system RelE/ParE family toxin -
KsCSTR_RS15045 3385526..3385714 - 189 WP_099326263.1 hypothetical protein -
KsCSTR_RS15050 3385788..3386405 - 618 WP_205713318.1 acyltransferase family protein -
KsCSTR_RS15055 3386578..3388101 + 1524 WP_076611933.1 IS1380-like element ISCku9 family transposase -
KsCSTR_RS15060 3388276..3388794 - 519 WP_164995333.1 acyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 3380589..3388101 7512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(18-72)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11222.65 Da        Isoelectric Point: 7.2446

>T150348 WP_099326265.1 NZ_CP049055:c3384235-3383942 [Candidatus Kuenenia stuttgartiensis]
VIQSFHDKGTEDIFDRKNTREARKTCPQQIWRAAQRKLDQLNGVVSLGSLKIPPGNMLEVLKDDRKGQHSIRINDQFRVC
FVWADDGVDNVEITDYH

Download         Length: 294 bp

>T150348 NZ_CP064678:c2904450-2904115 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 12152.29 Da        Isoelectric Point: 10.0019

>AT150348 WP_099326266.1 NZ_CP049055:c3383923-3383606 [Candidatus Kuenenia stuttgartiensis]
MVRIPKNGPPTHPGEMLLEEFLKPLHMTQRELAEKLGVSYPRVNELIHGKRGMTPDTALRLEKLFGMDAQFWLNLQLAWD
LYHVTHSSTAKEIQKIKRLPAFIHV

Download         Length: 318 bp

>AT150348 NZ_CP064678:c2904698-2904450 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References