Detailed information of TA system TA150348

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3383606..3384235
• Replicon: chromosome
• Accession: NZ_CP049055
• Organism: Candidatus Kuenenia stuttgartiensis strain CSTR1

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: KsCSTR_RS15030
• Protein ID: WP_099326265.1
• Coordinates: 3383942..3384235 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: KsCSTR_RS15025
• Protein ID: WP_099326266.1
• Coordinates: 3383606..3383923 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KsCSTR_RS15010	3380589..3382114	-	1526	Protein_2971	IS1380-like element ISCku9 family transposase	-
KsCSTR_RS15015	3382298..3382855	-	558	WP_157820667.1	GNAT family N-acetyltransferase	-
KsCSTR_RS15020	3383059..3383439	-	381	WP_099326267.1	hypothetical protein	-
KsCSTR_RS15025	3383606..3383923	-	318	WP_099326266.1	HigA family addiction module antidote protein	Antitoxin
KsCSTR_RS15030	3383942..3384235	-	294	WP_099326265.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KsCSTR_RS19765	3384840..3384956	-	117	WP_197705232.1	hypothetical protein	-
KsCSTR_RS15040	3385254..3385382	-	129	WP_197705231.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KsCSTR_RS15045	3385526..3385714	-	189	WP_099326263.1	hypothetical protein	-
KsCSTR_RS15050	3385788..3386405	-	618	WP_205713318.1	acyltransferase family protein	-
KsCSTR_RS15055	3386578..3388101	+	1524	WP_076611933.1	IS1380-like element ISCku9 family transposase	-
KsCSTR_RS15060	3388276..3388794	-	519	WP_164995333.1	acyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	3380589..3388101	7512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11222.65 Da
• Isoelectric Point: 7.2446

>T150348 WP_099326265.1 NZ_CP049055:c3384235-3383942 [Candidatus Kuenenia stuttgartiensis]
VIQSFHDKGTEDIFDRKNTREARKTCPQQIWRAAQRKLDQLNGVVSLGSLKIPPGNMLEVLKDDRKGQHSIRINDQFRVC
FVWADDGVDNVEITDYH

Nucleotide

• Length: 294 bp

>T150348 NZ_CP064678:c2904450-2904115 [Escherichia coli K-12]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 12152.29 Da
• Isoelectric Point: 10.0019

>AT150348 WP_099326266.1 NZ_CP049055:c3383923-3383606 [Candidatus Kuenenia stuttgartiensis]
MVRIPKNGPPTHPGEMLLEEFLKPLHMTQRELAEKLGVSYPRVNELIHGKRGMTPDTALRLEKLFGMDAQFWLNLQLAWD
LYHVTHSSTAKEIQKIKRLPAFIHV

Nucleotide

• Length: 318 bp

>AT150348 NZ_CP064678:c2904698-2904450 [Escherichia coli K-12]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA