Detailed information of TA system TA150346

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1104061..1104669
• Replicon: chromosome
• Accession: NZ_CP049055
• Organism: Candidatus Kuenenia stuttgartiensis strain CSTR1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KsCSTR_RS04835
• Protein ID: WP_164994611.1
• Coordinates: 1104388..1104669 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KsCSTR_RS04830
• Protein ID: WP_099324422.1
• Coordinates: 1104061..1104375 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KsCSTR_RS04805	1100335..1100493	+	159	WP_099324427.1	DUF3309 domain-containing protein	-
KsCSTR_RS04810	1100718..1101314	+	597	WP_164994610.1	hypothetical protein	-
KsCSTR_RS04815	1101676..1102530	+	855	WP_099324425.1	hypothetical protein	-
KsCSTR_RS04820	1102606..1102791	+	186	WP_099324424.1	hypothetical protein	-
KsCSTR_RS04825	1102918..1103694	+	777	WP_099324423.1	hypothetical protein	-
KsCSTR_RS04830	1104061..1104375	-	315	WP_099324422.1	HigA family addiction module antidote protein	Antitoxin
KsCSTR_RS04835	1104388..1104669	-	282	WP_164994611.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KsCSTR_RS04840	1104887..1105063	+	177	WP_164994612.1	hypothetical protein	-
KsCSTR_RS04845	1105101..1105805	+	705	WP_164995712.1	MBL fold metallo-hydrolase	-
KsCSTR_RS04850	1106153..1107835	+	1683	WP_205713375.1	IS1634 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (12-69)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11479.18 Da
• Isoelectric Point: 10.0997

>T150346 WP_164994611.1 NZ_CP049055:c1104669-1104388 [Candidatus Kuenenia stuttgartiensis]
MIKSFRCRETRKIFYREYSRRFPRDMQMRAFAKLNAIDAAIHLKDLHLPPSNRFEALKGERKEQYSIRINDRWRICFEWR
NGNAEEVEIVDYH

Nucleotide

• Length: 282 bp

>T150346 NZ_CP064678:2759277-2760350 [Escherichia coli K-12]
ATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATCCAGACAATTCATTGAAAATAA
AAACTATTCAATCCAATCAATTGGTCCTATGCCAGGGTCAAGGGCTGGGCTTCGGGTCGTATTTACCAGACCAGGGGTTA
ACTTGGCAACTGTGGACATTTTTTATAACGGGGACGGTTCGACTACAATTCAATATCTCACTGGAGCCAATCGTTCTCTG
GGCCAAGAGTTAGCGGATCATCTTTTTGAAACCATCAATCCTGCTGAATTTGAGCAGGTAAATATGGTACTGCAAGGATT
TGTAGAGACAAGCGTTCTACCTGTACTTGAGCTATCAGCAGATGAATCGCATATAGAGTTCAGAGAACACTCTCGTAACG
CTCATACCGTAGTGTGGAAAATTATTTCCACCAGCTATCAGGACGAATTGACTGTGAGCCTGCATATCACAACAGGTAAG
CTCCAGATTCAGGGCCGACCGCTGTCATGTTACAGAGTTTTCACGTTTAACTTGGCAGCCCTGCTTGATTTACAGGGTTT
GGAGAAAGTGCTAATCCGCCAGGAGGATGGTAAAGCTAATATTGTTCAACAGGAGGTTGCCCGCACTTACTTGCAGACTG
TAATGGCCGATGCTTACCCGCATCTCCACGTGACTGCCGAAAAATTGCTCGTTTCAGGGCTATGTGTTAAACTCGCCGCC
CCTGATTTGCCTGACTACTGTATGTTACTTTATCCTGAACTACGCACCATTGAAGGTGTCTTAAAAAGTAAGATGAGTGG
GTTAGGCATGCCAGTACAGCAGCCGGCAGGTTTTGGAACTTACTTTGATAAACCTGCTGCTCATTACATTCTGAAACCGC
AATTTGCAGCTACTCTTAGACCGGAACAGATTAACATCATCAGCACAGCCTATACTTTTTTTAATGTGGAACGTCATTCT
CTGTTCCACATGGAAACTGTGGTCGATGCCAGCCGTATGATTTCTGATATGGCCCGGTTGATGGGTAAAGCCACTAGAGC
GTGGGGAATAATCAAGGACTTATATATTGTTTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12280.28 Da
• Isoelectric Point: 10.4296

>AT150346 WP_099324422.1 NZ_CP049055:c1104375-1104061 [Candidatus Kuenenia stuttgartiensis]
MAKRRITPVHPGVYLKELLDELKISQYRLSQDLGVPAMRINHVVHSKRPVTAELALRLGRYFGQSPRYWMNLQSRYDMDI
AEDALFEQVTREVRPFNGRFIIHK

Nucleotide

• Length: 315 bp

>AT150346 NZ_CP064678:2760343-2760714 [Escherichia coli K-12]
TTGTTTGAAATCACCGGAATTAACGTCTCAGGGGCTCTAAAAGCTGTCGTTATGGCGACCGGCTTCGAGAACCCTTTGTC
CTCAGTTAATGAAATTGAGACGAAACTTTCTGCCCTACTGGGTAGTGAGACCACTGGTGAAATCCTTTTTGACCTCTTGT
GCGCTAACGGTCCAGAGTGGAACCGCTTCGTTACGCTGGAAATGAAATATGGCCGGATCATGCTGGATACGGCCAAAATT
ATTGATGAGCAGGACGTTCCGACACATATCCTGAGCAAACTGACTTTTACTTTACGTAATCATCCTGAGTACCTAGAAGC
CTCTGTTTTGTCTCCCGATGATGTACGCCAAGTCCTGTCAATGGATTTTTGA