Detailed information of TA system TA150338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3813341..3814071
• Replicon: chromosome
• Accession: NZ_CP049046
• Organism: Enterobacter hormaechei strain Y233

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: G5B34_RS18195
• Protein ID: WP_023302825.1
• Coordinates: 3813341..3813655 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5B34_RS18200
• Protein ID: WP_023302826.1
• Coordinates: 3813652..3814071 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5B34_RS18170	3809007..3810092	+	1086	Protein_3541	cellulase family glycosylhydrolase	-
G5B34_RS18175	3809998..3811182	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
G5B34_RS18180	3811358..3812191	-	834	WP_015570117.1	DMT family transporter	-
G5B34_RS18185	3812254..3812700	-	447	WP_164691978.1	GNAT family N-acetyltransferase	-
G5B34_RS18190	3812765..3812854	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
G5B34_RS18195	3813341..3813655	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
G5B34_RS18200	3813652..3814071	+	420	WP_023302826.1	helix-turn-helix domain-containing protein	Antitoxin
G5B34_RS18205	3814218..3814316	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
G5B34_RS18210	3814423..3816111	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
G5B34_RS18215	3816115..3816402	+	288	WP_015570113.1	acetolactate synthase small subunit	-
G5B34_RS18220	3816528..3817121	+	594	WP_003861054.1	transcriptional regulator UhpA	-
G5B34_RS18225	3817118..3818623	+	1506	WP_017694010.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T150338 WP_023302825.1 NZ_CP049046:3813341-3813655 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T150338 NZ_CP064678:c1408184-1407990 [Escherichia coli K-12]
ATGAGATATGACAATGTTAAACCATGTCCATTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
AGCGAAGTGTAACGGATGCGAATCCCGAACCGGTTATGGTGGAAGTGAAAAAGAAGCACTCGAAAGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.96 Da
• Isoelectric Point: 5.2068

>AT150338 WP_023302826.1 NZ_CP049046:3813652-3814071 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT150338 NZ_CP064678:1407813-1407991 [Escherichia coli K-12]
GAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTCGTTAGTTTAGATTTTGAATATCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A837FIL2