Detailed information of TA system TA150318

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 286943..287637
• Replicon: chromosome
• Accession: NZ_CP049044
• Organism: Pseudomonas psychrophila strain KM02

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8I1FWS5
• Locus tag: G5J76_RS01150
• Protein ID: WP_019825333.1
• Coordinates: 287350..287637 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5J76_RS01145
• Protein ID: WP_019825335.1
• Coordinates: 286943..287344 (-)
• Length: 134 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5J76_RS01135	281970..285317	+	3348	WP_048352363.1	mechanosensitive channel MscK	-
G5J76_RS01140	285470..286933	+	1464	WP_048352362.1	YdiU family protein	-
G5J76_RS01145	286943..287344	-	402	WP_019825335.1	transcriptional regulator	Antitoxin
G5J76_RS01150	287350..287637	-	288	WP_019825333.1	type II toxin-antitoxin system HigB family toxin	Toxin
G5J76_RS01155	287943..289739	+	1797	WP_048352361.1	SLC13 family permease	-
G5J76_RS01160	289792..290232	+	441	WP_019825330.1	thioredoxin TrxC	-
G5J76_RS01165	290229..291002	-	774	WP_019825328.1	ParA family protein	-
G5J76_RS01170	291340..292059	+	720	WP_046809139.1	beta-ketoacyl synthase chain length factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-90)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-130)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11306.06 Da
• Isoelectric Point: 10.5069

>T150318 WP_019825333.1 NZ_CP049044:c287637-287350 [Pseudomonas psychrophila]
MRVITAKRIWEAKEQWPQSASALDEWYRKIKVLKPEDFAALRSFFPATDKVGSFHVFNIGGNKLRLIAVVRYRVQRLYIR
HVLDHSDYDKGNWKE

Nucleotide

• Length: 288 bp

>T150318 NZ_CP064673:c45147-44767 [Salmonella sp. SJTUF14146]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 134 a.a.
• Molecular weight: 14952.13 Da
• Isoelectric Point: 4.4954

>AT150318 WP_019825335.1 NZ_CP049044:c287344-286943 [Pseudomonas psychrophila]
MNPLITQAAEHWKFVAPLLRKPKNEADYDELVAALDGLTDLIGDDESHPLMSLVDIIGDWVEAYDLEHRPMPKVSGVDVL
RSVMQEHGLTQSDLPGVGTQSVVSEILSGKRQLNVRQIRWLADYFKVPVDMFI

Nucleotide

• Length: 402 bp

>AT150318 NZ_CP064673:c45368-45147 [Salmonella sp. SJTUF14146]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA