Detailed information of TA system TA150312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3863137..3863812
• Replicon: chromosome
• Accession: NZ_CP049021
• Organism: Enterobacter hormaechei strain Eho-E3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI75_RS18740
• Protein ID: WP_032619177.1
• Coordinates: 3863137..3863436 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: GVI75_RS18745
• Protein ID: WP_015571639.1
• Coordinates: 3863447..3863812 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI75_RS18720	3858269..3858886	-	618	WP_032634496.1	DUF1819 family protein	-
GVI75_RS18725	3859050..3860018	-	969	WP_000654804.1	IS5-like element IS903B family transposase	-
GVI75_RS18730	3860051..3861706	+	1656	Protein_3667	ATP-binding cassette domain-containing protein	-
GVI75_RS18735	3861726..3862886	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GVI75_RS18740	3863137..3863436	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI75_RS18745	3863447..3863812	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
GVI75_RS18750	3863839..3865020	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
GVI75_RS18755	3865041..3865928	-	888	WP_032619173.1	LysR family transcriptional regulator	-
GVI75_RS18760	3866026..3866628	+	603	WP_017382759.1	short chain dehydrogenase	-
GVI75_RS18765	3866625..3867356	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3859050..3859973	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T150312 WP_032619177.1 NZ_CP049021:3863137-3863436 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T150312 NZ_CP064672:2503604-2503888 [Salmonella sp. SJTUF14146]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTACGGGAACGACTCGAAAACCCTCGGGTTCCTGCTTCGCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGGCACAGAAGCTAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT150312 WP_015571639.1 NZ_CP049021:3863447-3863812 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT150312 NZ_CP064672:2503366-2503614 [Salmonella sp. SJTUF14146]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9