Detailed information of TA system TA150267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3947518..3948212
• Replicon: chromosome
• Accession: NZ_CP048934
• Organism: Escherichia coli strain 190693

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: Q0T7Q5
• Locus tag: G5C00_RS18945
• Protein ID: WP_001263491.1
• Coordinates: 3947814..3948212 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: L4JHF0
• Locus tag: G5C00_RS18940
• Protein ID: WP_000554755.1
• Coordinates: 3947518..3947811 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C00_RS18915	3943139..3943495	-	357	WP_001030484.1	type II toxin-antitoxin system HicB family antitoxin	-
G5C00_RS18920	3943488..3943766	-	279	WP_000598760.1	type II toxin-antitoxin system HicA family toxin	-
G5C00_RS18925	3943871..3945583	-	1713	Protein_3680	flagellar biosynthesis protein FlhA	-
G5C00_RS18930	3945555..3946340	+	786	WP_019842510.1	putative lateral flagellar export/assembly protein LafU	-
G5C00_RS18935	3946411..3947466	+	1056	WP_001550655.1	DNA polymerase IV	-
G5C00_RS18940	3947518..3947811	+	294	WP_000554755.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G5C00_RS18945	3947814..3948212	+	399	WP_001263491.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G5C00_RS18950	3948222..3948674	+	453	WP_019842500.1	GNAT family N-acetyltransferase	-
G5C00_RS18955	3948920..3949126	+	207	Protein_3686	RtcB family protein	-
G5C00_RS18960	3949122..3949474	+	353	Protein_3687	peptide chain release factor H	-
G5C00_RS18965	3949531..3950988	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
G5C00_RS18970	3951249..3951707	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G5C00_RS18975	3951799..3953043	+	1245	WP_000189541.1	esterase FrsA	-
-	3952303..3952383	+	81	NuclAT_10	-	-
-	3952303..3952383	+	81	NuclAT_10	-	-
-	3952303..3952383	+	81	NuclAT_10	-	-
-	3952303..3952383	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15457.86 Da
• Isoelectric Point: 8.0949

>T150267 WP_001263491.1 NZ_CP048934:3947814-3948212 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150267 NZ_CP064661:2449266-2449550 [Salmonella sp. SJTUF14178]
ATGACTTATAAGCTGGCATTTAACGAATCTGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTACGGGAACGACTCGAAAACCCTCGGGTTCCTGCTTCGCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGGCACAGAAGCTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11235.78 Da
• Isoelectric Point: 5.2170

>AT150267 WP_000554755.1 NZ_CP048934:3947518-3947811 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150267 NZ_CP064661:2449028-2449276 [Salmonella sp. SJTUF14178]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGACCCAATGGGAACTTTCAATGCCGG
AGATGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: A0A4P7TPK7

Antitoxin

• AlphaFold DB: A0A829G9H5