Detailed information of TA system TA150263

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3037589..3038191
• Replicon: chromosome
• Accession: NZ_CP048934
• Organism: Escherichia coli strain 190693

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G5C00_RS14670
• Protein ID: WP_000897305.1
• Coordinates: 3037589..3037900 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G5C00_RS14675
• Protein ID: WP_000356395.1
• Coordinates: 3037901..3038191 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5C00_RS14650	3032631..3033068	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G5C00_RS14655	3033113..3034054	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G5C00_RS14660	3034470..3036284	+	1815	WP_001550352.1	hypothetical protein	-
G5C00_RS14665	3036452..3037360	-	909	WP_001550353.1	alpha/beta hydrolase	-
G5C00_RS14670	3037589..3037900	+	312	WP_000897305.1	hypothetical protein	Toxin
G5C00_RS14675	3037901..3038191	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
G5C00_RS14680	3038276..3038497	+	222	WP_001550354.1	hypothetical protein	-
G5C00_RS14685	3038549..3038827	+	279	WP_001315112.1	hypothetical protein	-
G5C00_RS14690	3039246..3039464	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
G5C00_RS14695	3039683..3039925	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
G5C00_RS14700	3040107..3041036	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
G5C00_RS14705	3041033..3041668	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G5C00_RS14710	3041665..3042567	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T150263 WP_000897305.1 NZ_CP048934:3037589-3037900 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T150263 NZ_CP064661:851066-851464 [Salmonella sp. SJTUF14178]
ATGCTGAAATTCATGCTTGATACCAATACCTGTATTTTCACCATCAAAAATAAGCCCGAACACATCAGAGAACGCTTCAA
CCTCAATACATCCCGAATGTGTATCAGCTCCATCACCTTAATGGAGCTGATTTACGGTGCTGAAAAAAGCCTGGCGCCGG
AGCGTAATCTTGCCGTCGTGGAGGGATTTATCTCCCGCCTTGAGGTTCTGGATTACGATACACAGGCAGCGATACATACC
GGTCAAATCCGTGCCGAACTGGCCCGCAAGGGAACACCTGTCGGGCCTTATGACCAGATGATTGCTGGCCATGCCCGTAG
CCGCGGACTGGTCGTCGTCACAAACAATCTCCGCGAGTTTGAACGCATTCCGGGTATCCGAATCGAAGACTGGTGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT150263 WP_000356395.1 NZ_CP048934:3037901-3038191 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT150263 NZ_CP064661:850839-851066 [Salmonella sp. SJTUF14178]
ATGCACACAACACTTTTTTTTAGTAACCGGACCCAGGCAGTCAGATTGCCTAAATCGATATCCTTTCCGGATGATGTGAA
ACATGTCGAAATCATTGCGGTTGGTAGAAGCAGAATCATTACTCCAGTCGGAGAATCCTGGGACAGCTGGTTCGATGGCG
AAGGCGCCAGTACTGATTTTATGAGTACCAGAGAACAACCCGCAGTACAGGAACGGGAAGGATTCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7