Detailed information of TA system TA150197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 685395..686194
• Replicon: chromosome
• Accession: NZ_CP048920
• Organism: Escherichia coli strain SCU-101

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q8FDB4
• Locus tag: G4235_RS03335
• Protein ID: WP_000347252.1
• Coordinates: 685395..685859 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1PPV5
• Locus tag: G4235_RS03340
• Protein ID: WP_001296435.1
• Coordinates: 685859..686194 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4235_RS03305	680396..680830	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
G4235_RS03310	680848..681726	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
G4235_RS03315	681716..682495	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
G4235_RS03320	682506..682979	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
G4235_RS03325	683002..684282	-	1281	WP_000681926.1	tagatose-bisphosphate aldolase subunit KbaZ	-
G4235_RS03330	684531..685340	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
G4235_RS03335	685395..685859	-	465	WP_000347252.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
G4235_RS03340	685859..686194	-	336	WP_001296435.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
G4235_RS03345	686343..687914	-	1572	WP_001273940.1	galactarate dehydratase	-
G4235_RS03350	688289..689623	+	1335	WP_000979025.1	galactarate/glucarate/glycerate transporter GarP	-
G4235_RS03355	689639..690409	+	771	WP_001058223.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17750.11 Da
• Isoelectric Point: 9.4947

>T150197 WP_000347252.1 NZ_CP048920:c685859-685395 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEESH

Nucleotide

• Length: 465 bp

>T150197 NZ_CP064405:717830-718225 [Mycobacterium tuberculosis variant bovis BCG]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12321.92 Da
• Isoelectric Point: 4.5392

>AT150197 WP_001296435.1 NZ_CP048920:c686194-685859 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT150197 NZ_CP064405:717576-717830 [Mycobacterium tuberculosis variant bovis BCG]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: Q8FDB4

Antitoxin

• AlphaFold DB: S1PPV5