Detailed information of TA system TA150191

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 69155..69394
• Replicon: plasmid pE2-NDM-CTX-M
• Accession: NZ_CP048916
• Organism: Escherichia coli strain E2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: G5S48_RS23190
• Protein ID: WP_023144756.1
• Coordinates: 69155..69289 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	69334..69394 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G5S48_RS23160 (64856)	64856..65713	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
G5S48_RS23165 (65706)	65706..65780	-	75	WP_031943482.1	RepA leader peptide Tap	-
G5S48_RS23175 (66142)	66142..67683	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
G5S48_RS23180 (67698)	67698..68444	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
G5S48_RS23185 (68604)	68604..68858	-	255	WP_000083850.1	replication regulatory protein RepA	-
G5S48_RS23190 (69155)	69155..69289	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (69334)	69334..69394	+	61	NuclAT_0	-	Antitoxin
- (69334)	69334..69394	+	61	NuclAT_0	-	Antitoxin
- (69334)	69334..69394	+	61	NuclAT_0	-	Antitoxin
- (69334)	69334..69394	+	61	NuclAT_0	-	Antitoxin
G5S48_RS23195 (69361)	69361..69647	-	287	Protein_87	DUF2726 domain-containing protein	-
G5S48_RS23200 (70160)	70160..70372	-	213	WP_013023861.1	hypothetical protein	-
G5S48_RS23205 (70503)	70503..71063	-	561	WP_000139341.1	fertility inhibition protein FinO	-
G5S48_RS23210 (71118)	71118..71864	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
G5S48_RS23215 (71884)	71884..74277	-	2394	Protein_91	conjugative transfer relaxase/helicase TraI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / aadA5 / blaNDM-5 / aadA2 / dfrA12 / blaTEM-1B / aac(3)-IId / mph(A) / aph(6)-Id / aph(3'')-Ib / sul2 / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..111334	111334

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T150191 WP_023144756.1 NZ_CP048916:c69289-69155 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T150191 NZ_CP064405:c445957-445364 [Mycobacterium tuberculosis variant bovis BCG]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAACAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Nucleotide

• Length: 61 bp

>AT150191 NZ_CP048916:69334-69394 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin