Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 629557..630356 Replicon chromosome
Accession NZ_CP048915
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag G5S48_RS03060 Protein ID WP_000347251.1
Coordinates 629557..630021 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G5S48_RS03065 Protein ID WP_001307405.1
Coordinates 630021..630356 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G5S48_RS03030 (624558) 624558..624992 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
G5S48_RS03035 (625010) 625010..625888 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
G5S48_RS03040 (625878) 625878..626657 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
G5S48_RS03045 (626668) 626668..627141 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G5S48_RS03050 (627164) 627164..628444 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
G5S48_RS03055 (628693) 628693..629502 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G5S48_RS03060 (629557) 629557..630021 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G5S48_RS03065 (630021) 630021..630356 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G5S48_RS03070 (630505) 630505..632076 - 1572 WP_001273761.1 galactarate dehydratase -
G5S48_RS03075 (632451) 632451..633785 + 1335 WP_000599652.1 galactarate/glucarate/glycerate transporter GarP -
G5S48_RS03080 (633801) 633801..634571 + 771 WP_001058214.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T150171 WP_000347251.1 NZ_CP048915:c630021-629557 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T150171 NZ_CP064401:c4726279-4725869 [Pseudomonas aeruginosa]
ATGCTCAAGTTCATGCTTGACACCAACATCTGCATCTTCACCATCAAAAACCGCCCGCAAGAGGTGCGCGAGGCGTTCCT
GCGCCACCACGACCAGATGTGCATCAGCACCGTGACGCTGATGGAGCTGATCTACGGCGCAGAGAAGTCCTCGAACCCCT
CGCGCAATCTTGCAGACGTGGAAGGCTTCGCTGCTCGTCTGGAGGTGCTCAAGTACGACCAGGAAGCTGCCGCCCACACC
GGCCAGCTCCGCGCTGAACTGGCCCGCCTCGGCAAGCAAATCGGCCCCTATGACCAGATGATCGCCGGGCACGCCCGCTC
TCAGGGCCTGATCGTGGTCACCAATAACCGCCGCGAGTTCGACCGCGTACCCGGCTTGAGAGTTGAGGATTGGGTCACCC
CAATCGTGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT150171 WP_001307405.1 NZ_CP048915:c630356-630021 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT150171 NZ_CP064401:c4726509-4726279 [Pseudomonas aeruginosa]
GTGGAACAAGGTTCTGTTTTTCAGAGCAATCGTAGCCAGGCCGTAAGACTGCCCAAGGCCGTCGCGCTTCCTGATGACGT
TAAGCGTGTGGATGTGGTCGCTGTGGGGCGCGCCAGAATCATCACGCCTGCCGGCGAGTCTTGGGATAGCTGGTTCGATG
GCGAGGGCGTTACTGCCGACTTTATGGCAGAGCGTGAACAGCCTGCCGATCAGGAGCGCGAGACTTTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References