Detailed information of TA system TA150087

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 64637..64901
• Replicon: plasmid pEcQE11-421-3
• Accession: NZ_CP048824
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: QE11421_RS24210
• Protein ID: WP_001303307.1
• Coordinates: 64749..64901 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	64637..64699 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS24195	60739..61809	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
QE11421_RS24200	61828..63036	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	63216..63276	-	61	NuclAT_1	-	-
-	63216..63276	-	61	NuclAT_1	-	-
-	63216..63276	-	61	NuclAT_1	-	-
-	63216..63276	-	61	NuclAT_1	-	-
QE11421_RS24205	63343..64434	-	1092	WP_000426061.1	hypothetical protein	-
-	64637..64699	-	63	NuclAT_0	-	Antitoxin
-	64637..64699	-	63	NuclAT_0	-	Antitoxin
-	64637..64699	-	63	NuclAT_0	-	Antitoxin
-	64637..64699	-	63	NuclAT_0	-	Antitoxin
QE11421_RS24210	64749..64901	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
QE11421_RS24215	64973..65224	-	252	WP_001291965.1	hypothetical protein	-
-	65611..65662	-	52	NuclAT_2	-	-
-	65611..65662	-	52	NuclAT_2	-	-
-	65611..65662	-	52	NuclAT_2	-	-
-	65611..65662	-	52	NuclAT_2	-	-
QE11421_RS24220	66148..66324	-	177	WP_001054900.1	hypothetical protein	-
QE11421_RS24225	66533..66742	-	210	Protein_71	hemolysin expression modulator Hha	-
QE11421_RS24230	66840..67454	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
QE11421_RS24235	67530..69698	-	2169	WP_199266157.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib	-	1..110687	110687

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T150087 WP_001303307.1 NZ_CP048824:64749-64901 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T150087 NZ_CP064387:2280982-2281200 [Escherichia coli O104:H4]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT150087 NZ_CP048824:c64699-64637 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin