Detailed information of TA system TA150082

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 112427..112853
• Replicon: plasmid pEcQE11-421-1
• Accession: NZ_CP048822
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: QE11421_RS23105
• Protein ID: WP_001312861.1
• Coordinates: 112427..112585 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	112629..112853 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS23075	107448..107843	-	396	WP_001309237.1	conjugal transfer relaxosome protein TraY	-
QE11421_RS23080	107942..108631	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
QE11421_RS23085	108818..109201	-	384	WP_001151524.1	relaxosome protein TraM	-
QE11421_RS23090	109522..110124	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
QE11421_RS23095	110421..111242	-	822	WP_001234469.1	DUF945 domain-containing protein	-
QE11421_RS23100	111364..111651	-	288	WP_000107535.1	hypothetical protein	-
QE11421_RS23105	112427..112585	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	112629..112853	-	225	NuclAT_0	-	Antitoxin
-	112629..112853	-	225	NuclAT_0	-	Antitoxin
-	112629..112853	-	225	NuclAT_0	-	Antitoxin
-	112629..112853	-	225	NuclAT_0	-	Antitoxin
QE11421_RS23110	112665..112853	+	189	WP_001299721.1	hypothetical protein	-
QE11421_RS23115	112865..113584	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
QE11421_RS23120	113581..114015	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
QE11421_RS23125	114070..116028	-	1959	WP_089503137.1	ParB/RepB/Spo0J family partition protein	-
QE11421_RS23130	116087..116320	-	234	WP_000006004.1	DUF905 domain-containing protein	-
QE11421_RS23135	116376..116903	-	528	WP_000290793.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul2 / aph(3'')-Ib / aph(6)-Id / blaTEM-1B / sitABCD	iroB / iroC / iroD / iroE / iroN / vat	1..137410	137410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T150082 WP_001312861.1 NZ_CP048822:c112585-112427 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T150082 NZ_CP064387:1276460-1276567 [Escherichia coli O104:H4]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGGATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT150082 NZ_CP048822:c112853-112629 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin