Detailed information of TA system TA150078

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3955470..3956164
• Replicon: chromosome
• Accession: NZ_CP048821
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: QE11421_RS19210
• Protein ID: WP_001263493.1
• Coordinates: 3955766..3956164 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: QE11421_RS19205
• Protein ID: WP_000554757.1
• Coordinates: 3955470..3955763 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS19180	3951284..3951751	+	468	WP_000725257.1	flagellar basal body-associated FliL family protein	-
QE11421_RS19185	3951771..3952490	+	720	WP_000938740.1	FliA/WhiG family RNA polymerase sigma factor	-
QE11421_RS19190	3952503..3953366	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
QE11421_RS19195	3953369..3954292	+	924	WP_001468020.1	putative lateral flagellar export/assembly protein LafU	-
QE11421_RS19200	3954363..3955418	+	1056	WP_001226164.1	DNA polymerase IV	-
QE11421_RS19205	3955470..3955763	+	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
QE11421_RS19210	3955766..3956164	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
QE11421_RS19215	3956174..3956626	+	453	WP_001059847.1	GNAT family N-acetyltransferase	-
QE11421_RS19220	3956872..3957075	+	204	Protein_3742	RNA ligase RtcB family protein	-
QE11421_RS19225	3957074..3957595	+	522	Protein_3743	peptide chain release factor H	-
QE11421_RS19230	3957652..3959109	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
QE11421_RS19235	3959370..3959828	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
QE11421_RS19240	3959920..3961164	+	1245	WP_000189541.1	esterase FrsA	-
-	3960424..3960504	+	81	NuclAT_10	-	-
-	3960424..3960504	+	81	NuclAT_10	-	-
-	3960424..3960504	+	81	NuclAT_10	-	-
-	3960424..3960504	+	81	NuclAT_10	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T150078 WP_001263493.1 NZ_CP048821:3955766-3956164 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T150078 NZ_CP064387:255943-256194 [Escherichia coli O104:H4]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT150078 WP_000554757.1 NZ_CP048821:3955470-3955763 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT150078 NZ_CP064387:256191-256445 [Escherichia coli O104:H4]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6