Detailed information of TA system TA150070

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/YoeB-YefM
• Location: 1108465..1108967
• Replicon: chromosome
• Accession: NZ_CP048821
• Organism: Escherichia coli strain QEC11-421

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: U9YDH0
• Locus tag: QE11421_RS05565
• Protein ID: WP_000767829.1
• Coordinates: 1108465..1108719 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: S1Q063
• Locus tag: QE11421_RS05570
• Protein ID: WP_001259255.1
• Coordinates: 1108716..1108967 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QE11421_RS05535	1103480..1103707	-	228	WP_000985273.1	sulfurtransferase TusA family protein	-
QE11421_RS05540	1103721..1104779	-	1059	WP_000492327.1	transport protein YeeE	-
QE11421_RS05545	1104958..1106316	-	1359	WP_000019197.1	putrescine/proton symporter PlaP	-
QE11421_RS05550	1106306..1106368	-	63	WP_010723108.1	membrane protein YoeI	-
QE11421_RS05555	1106583..1107512	-	930	WP_000803366.1	LysR family transcriptional regulator	-
QE11421_RS05560	1107558..1108382	-	825	WP_000754752.1	SDR family oxidoreductase	-
QE11421_RS05565	1108465..1108719	-	255	WP_000767829.1	type II toxin-antitoxin system mRNA interferase toxin YoeB	Toxin
QE11421_RS05570	1108716..1108967	-	252	WP_001259255.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
QE11421_RS05575	1109251..1109301	+	51	WP_001364200.1	his operon leader peptide	-
QE11421_RS05580	1109447..1110346	+	900	WP_000131782.1	ATP phosphoribosyltransferase	-
QE11421_RS05585	1110352..1111656	+	1305	WP_039023537.1	histidinol dehydrogenase	-
QE11421_RS05590	1111653..1112723	+	1071	WP_039023536.1	histidinol-phosphate transaminase	-
QE11421_RS05595	1112723..1113790	+	1068	WP_000080077.1	bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (2-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10183.58 Da
• Isoelectric Point: 8.0353

>T150070 WP_000767829.1 NZ_CP048821:c1108719-1108465 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Nucleotide

• Length: 255 bp

>T150070 NZ_CP064385:2987270-2987581 [Salmonella enterica subsp. enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9307.58 Da
• Isoelectric Point: 4.7954

>AT150070 WP_001259255.1 NZ_CP048821:c1108967-1108716 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Nucleotide

• Length: 252 bp

>AT150070 NZ_CP064385:2987582-2987872 [Salmonella enterica subsp. enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Toxin

• PDB: 6NY6
• PDB: 2A6S
• PDB: 6OXA
• PDB: 2A6R
• PDB: 4V8X
• PDB: 6OTR
• PDB: 6OXI
• PDB: 2A6Q

Antitoxin

• PDB: 2A6Q
• AlphaFold DB: A0A7U9LNR0