150047

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3035033..3035721 Replicon chromosome
Accession NZ_CP048814
Organism Rhodococcus triatomae strain DSM 44892

Toxin (Protein)


Gene name higB Uniprot ID A0A1G8NNJ2
Locus tag G4H71_RS14305 Protein ID WP_072738929.1
Coordinates 3035033..3035386 (+) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1G8NNF1
Locus tag G4H71_RS14310 Protein ID WP_072739026.1
Coordinates 3035407..3035721 (+) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4H71_RS14280 3030294..3031142 + 849 WP_072738926.1 glycoside hydrolase -
G4H71_RS14285 3031557..3031766 + 210 WP_072738927.1 heavy-metal-associated domain-containing protein -
G4H71_RS14290 3031819..3032007 + 189 WP_072738928.1 hypothetical protein -
G4H71_RS14295 3032089..3034272 + 2184 WP_072739024.1 heavy metal translocating P-type ATPase -
G4H71_RS14300 3034577..3034924 + 348 WP_072739025.1 metal-sensitive transcriptional regulator -
G4H71_RS14305 3035033..3035386 + 354 WP_072738929.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G4H71_RS14310 3035407..3035721 + 315 WP_072739026.1 helix-turn-helix transcriptional regulator Antitoxin
G4H71_RS14315 3035887..3036423 - 537 WP_083343226.1 DUF2269 domain-containing protein -
G4H71_RS14320 3036420..3036680 - 261 WP_072738930.1 hypothetical protein -
G4H71_RS14325 3036779..3037348 + 570 WP_072738931.1 TetR/AcrR family transcriptional regulator -
G4H71_RS14330 3037833..3038157 - 325 Protein_2824 thioredoxin family protein -
G4H71_RS14335 3038178..3039098 - 921 WP_083343216.1 metalloregulator ArsR/SmtB family transcription factor -
G4H71_RS14340 3039160..3040500 + 1341 WP_072738933.1 NAD(P)-binding domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(21-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-90)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13492.32 Da        Isoelectric Point: 4.3715

>T150047 WP_072738929.1 NZ_CP048814:3035033-3035386 [Rhodococcus triatomae]
MECEVVLLDDVEQWYLRLVESDDGDAAAVTAAIDYLEREGPTLGRPVVDKVKGSRVHNMKELRPSGTSIRILFVFDPQRQ
AVLLIAGDKAGEWKQWYIDNIPIAEDRYDMWLEEQKR

Download         Length: 354 bp

>T150047 NZ_CP064383:c3465902-3465495 [Escherichia coli O157:H7]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11796.22 Da        Isoelectric Point: 6.4886

>AT150047 WP_072739026.1 NZ_CP048814:3035407-3035721 [Rhodococcus triatomae]
MARHWREVRAEVAEKLDEQAVSNASKTMQEEVRAHRLVEIRREQGWTRQDAVAEVMGISQSRVSQIERGDLRHTQLGTLE
SYVEALGGHLRVVADFGDRSLTLG

Download         Length: 315 bp

>AT150047 NZ_CP064383:c3466149-3465883 [Escherichia coli O157:H7]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCCGATC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G8NNJ2

Antitoxin

Source ID Structure
AlphaFold DB A0A1G8NNF1

References