Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 3460791..3461479 Replicon chromosome
Accession NZ_CP048813
Organism Rhodococcus triatomae strain DSM 44893

Toxin (Protein)


Gene name higB Uniprot ID A0A1G8NNJ2
Locus tag G4H72_RS16070 Protein ID WP_072738929.1
Coordinates 3461126..3461479 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A1G8NNF1
Locus tag G4H72_RS16065 Protein ID WP_072739026.1
Coordinates 3460791..3461105 (-) Length 105 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4H72_RS16035 3456013..3457353 - 1341 WP_072738933.1 NAD(P)-binding domain-containing protein -
G4H72_RS16040 3457415..3458335 + 921 WP_083343216.1 metalloregulator ArsR/SmtB family transcription factor -
G4H72_RS16045 3458356..3458679 + 324 WP_072739028.1 thioredoxin family protein -
G4H72_RS16050 3459164..3459733 - 570 WP_072738931.1 TetR/AcrR family transcriptional regulator -
G4H72_RS16055 3459832..3460092 + 261 WP_072738930.1 hypothetical protein -
G4H72_RS16060 3460089..3460625 + 537 WP_083343226.1 DUF2269 domain-containing protein -
G4H72_RS16065 3460791..3461105 - 315 WP_072739026.1 helix-turn-helix transcriptional regulator Antitoxin
G4H72_RS16070 3461126..3461479 - 354 WP_072738929.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G4H72_RS16075 3461588..3461935 - 348 WP_072739025.1 metal-sensitive transcriptional regulator -
G4H72_RS16080 3462240..3464423 - 2184 WP_072739024.1 heavy metal translocating P-type ATPase -
G4H72_RS16085 3464505..3464693 - 189 WP_072738928.1 hypothetical protein -
G4H72_RS16090 3464746..3464955 - 210 WP_072738927.1 heavy-metal-associated domain-containing protein -
G4H72_RS16095 3465370..3466218 - 849 WP_072738926.1 glycoside hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(21-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(34-90)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13492.32 Da        Isoelectric Point: 4.3715

>T150043 WP_072738929.1 NZ_CP048813:c3461479-3461126 [Rhodococcus triatomae]
MECEVVLLDDVEQWYLRLVESDDGDAAAVTAAIDYLEREGPTLGRPVVDKVKGSRVHNMKELRPSGTSIRILFVFDPQRQ
AVLLIAGDKAGEWKQWYIDNIPIAEDRYDMWLEEQKR

Download         Length: 354 bp

>T150043 NZ_CP064383:c1680008-1679832 [Escherichia coli O157:H7]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 105 a.a.        Molecular weight: 11796.22 Da        Isoelectric Point: 6.4886

>AT150043 WP_072739026.1 NZ_CP048813:c3461105-3460791 [Rhodococcus triatomae]
MARHWREVRAEVAEKLDEQAVSNASKTMQEEVRAHRLVEIRREQGWTRQDAVAEVMGISQSRVSQIERGDLRHTQLGTLE
SYVEALGGHLRVVADFGDRSLTLG

Download         Length: 315 bp

>AT150043 NZ_CP064383:c1679786-1679370 [Escherichia coli O157:H7]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAAGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCAGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1G8NNJ2

Antitoxin

Source ID Structure
AlphaFold DB A0A1G8NNF1

References