Detailed information of TA system TA150033

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 4807107..4807779
• Replicon: chromosome
• Accession: NZ_CP048810
• Organism: Pseudomonas bijieensis strain L22-9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GN234_RS21050
• Protein ID: WP_176689018.1
• Coordinates: 4807107..4807472 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GN234_RS21055
• Protein ID: WP_109754986.1
• Coordinates: 4807465..4807779 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN234_RS21040	4804257..4805051	-	795	WP_176689016.1	carbon-nitrogen hydrolase family protein	-
GN234_RS21045	4805064..4806746	-	1683	WP_176689017.1	FAD-dependent oxidoreductase	-
GN234_RS21050	4807107..4807472	+	366	WP_176689018.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN234_RS21055	4807465..4807779	+	315	WP_109754986.1	XRE family transcriptional regulator	Antitoxin
GN234_RS21060	4807802..4808263	-	462	WP_018607342.1	Lrp/AsnC family transcriptional regulator	-
GN234_RS21065	4808300..4810624	-	2325	WP_176689615.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-106)

Antitoxin

• HigA2-like, helix-turn-helix domain (16-86)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13987.65 Da
• Isoelectric Point: 5.2052

>T150033 WP_176689018.1 NZ_CP048810:4807107-4807472 [Pseudomonas bijieensis]
MKWDVEYTDEFEMWWSCLSETEQDSVQASVMLLGDAGPHLGFPHTSDIKGSRHGNLRELRVQHAGRPYRVLHAFDPRRCA
ILLIGGDKTGQDRWYQECVPLAERLYDEHLEALKREGFDNG

Nucleotide

• Length: 366 bp

>T150033 NZ_CP064377:4898280-4898855 [Achromobacter xylosoxidans]
ATGAGGCATTCCCCCTTCACAGCCGTCTACGACGCATGCGTTTTGTATCCCGCGCCACTGCGGGACTTCCTGATGTGGCT
CGGCCTGTCCGGCCGCTTCAGGGCGCGGTGGAGTCAAGCCATCCATGAGGAGTGGAAACGCAACCTGTTGATCAAACGAC
CCGATCTCAACCGGGCGCAGGTCGACAGGACGTCCGATCTCATGGACAGGGCCATTCCGGACGGCGTGGTGGAGGGATAT
GAAGAGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGATCGGCACGTCCTGGCTGCGGCGATCCGCTGTGGTGCGAG
CGTGATCGTGACGTTCAACGAACGAGATTTCCCGAACGAGCTGCTGGCTGCGTACGGTATCGAAGCGCAGCACCCCGACG
AGTTCGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTCGTGTCAGCAGCGCAGCGCCAGCGTGCCCAGCTCAAGCAT
CCGCCAATCGACGTGGACTGCTTTCTCGAAATCCTGCTTCGCCAAGGCTTGGTGCAAACGACCAAAGTGCTGGCGACCTA
TCGCACCATCCTCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12002.75 Da
• Isoelectric Point: 9.9863

>AT150033 WP_109754986.1 NZ_CP048810:4807465-4807779 [Pseudomonas bijieensis]
MAKKFSDLIARRSPDSRARAETLYQKLRAEMPLHELRQAQELSQDALAKTLHINQAAVSKMERRTDMYISTLRNYIRAMG
GELEIIATFPEGQVRIENFAHQTQ

Nucleotide

• Length: 315 bp

>AT150033 NZ_CP064377:4897813-4898283 [Achromobacter xylosoxidans]
ATGACCACCACCGCTGCCCAATCCAAGATGACCCTTCCCGCTGCGGCAGAGGTGAAGGCCGCCGTTCAGGGTCAACGTGC
CTTGGCGGCCTATCTGGCAACCCAGTTCGAGACGCAGCACATTCAGATCTTTGACGACCACAAGCAGGCGCATCAGGTGG
AGCTGCCCACCTCGGCTTTGCGCCTGCTTGTCGACATCCTGGCTGAACTGGCCGATGGCAACGCCGTGAAGGTGGTGCCC
GTTCACGCAGAGCTGACGACACAGGAGGCAGCCGATCTGCTCAACGTGTCCCGCCCCCATTTAGTCAAGCTGCTCGAGGA
TGGAGTGTTGGCATTTCACCGGACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGGTACGAATGA