Detailed information of TA system TA150030

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1960299..1960903
• Replicon: chromosome
• Accession: NZ_CP048810
• Organism: Pseudomonas bijieensis strain L22-9

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GN234_RS08340
• Protein ID: WP_116831887.1
• Coordinates: 1960299..1960613 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N1CCA5
• Locus tag: GN234_RS08345
• Protein ID: WP_058545613.1
• Coordinates: 1960616..1960903 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GN234_RS08320	1955761..1957332	-	1572	WP_116831890.1	GGDEF domain-containing protein	-
GN234_RS08325	1957544..1958203	-	660	WP_176688257.1	ribonuclease T2	-
GN234_RS08330	1958382..1959740	+	1359	WP_176688258.1	chromate efflux transporter	-
GN234_RS08335	1959737..1960168	-	432	WP_176688259.1	GNAT family N-acetyltransferase	-
GN234_RS08340	1960299..1960613	+	315	WP_116831887.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GN234_RS08345	1960616..1960903	+	288	WP_058545613.1	helix-turn-helix domain-containing protein	Antitoxin
GN234_RS08350	1961047..1961532	+	486	WP_176688260.1	hypothetical protein	-
GN234_RS08355	1961548..1961937	-	390	WP_176689569.1	helix-turn-helix transcriptional regulator	-
GN234_RS08360	1962034..1962441	+	408	WP_163854612.1	DoxX family protein	-
GN234_RS08365	1962453..1963670	-	1218	WP_176688261.1	MFS transporter	-
GN234_RS08370	1963797..1964846	-	1050	WP_176688262.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11934.03 Da
• Isoelectric Point: 10.1359

>T150030 WP_116831887.1 NZ_CP048810:1960299-1960613 [Pseudomonas bijieensis]
MIFIETPIFTRRVKELMDDDDFAVLQKILVCNPSAGDVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKLIVEHWR

Nucleotide

• Length: 315 bp

>T150030 NZ_CP064376:4881049-4881384 [Shigella sonnei]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.40 Da
• Isoelectric Point: 8.7885

>AT150030 WP_058545613.1 NZ_CP048810:1960616-1960903 [Pseudomonas bijieensis]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Nucleotide

• Length: 288 bp

>AT150030 NZ_CP064376:4880801-4881049 [Shigella sonnei]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6N1CCA5