Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 81640..82314 Replicon plasmid pEGKP4.1
Accession NZ_CP048801
Organism Klebsiella pneumoniae strain EGKP4

Toxin (Protein)


Gene name higB Uniprot ID A0A5Q9LMS5
Locus tag G4I41_RS26715 Protein ID WP_032720638.1
Coordinates 81640..81963 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag G4I41_RS26720 Protein ID WP_032720637.1
Coordinates 82012..82314 (+) Length 101 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G4I41_RS26680 (G4I41_27125) 76916..77281 - 366 WP_001294656.1 mercuric ion transporter MerT -
G4I41_RS26685 (G4I41_27130) 77353..77808 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
G4I41_RS26690 (G4I41_27135) 77842..78390 + 549 Protein_87 SOS response-associated peptidase family protein -
G4I41_RS26695 (G4I41_27140) 78786..79483 + 698 WP_223367361.1 IS1 family transposase -
G4I41_RS26700 (G4I41_27145) 79981..80583 + 603 Protein_89 RNA-guided endonuclease TnpB family protein -
G4I41_RS26705 80652..81079 - 428 Protein_90 DNA-binding protein -
G4I41_RS26710 (G4I41_27150) 81057..81462 + 406 Protein_91 DUF4113 domain-containing protein -
G4I41_RS26715 (G4I41_27155) 81640..81963 + 324 WP_032720638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G4I41_RS26720 (G4I41_27160) 82012..82314 + 303 WP_032720637.1 NadS family protein Antitoxin
G4I41_RS26725 (G4I41_27165) 82357..82524 - 168 WP_153591440.1 hypothetical protein -
G4I41_RS26730 (G4I41_27170) 82712..82918 - 207 WP_153591439.1 hypothetical protein -
G4I41_RS26735 (G4I41_27175) 82915..83298 - 384 WP_032720636.1 hypothetical protein -
G4I41_RS26740 (G4I41_27180) 83403..83975 - 573 WP_032720635.1 GNAT family N-acetyltransferase -
G4I41_RS26745 (G4I41_27185) 83977..84789 - 813 WP_223203129.1 TSUP family transporter -
G4I41_RS26750 (G4I41_27190) 84801..85721 - 921 WP_050548604.1 TauD/TfdA family dioxygenase -
G4I41_RS26755 (G4I41_27195) 86024..86518 - 495 WP_044266755.1 hypothetical protein -
G4I41_RS26760 (G4I41_27200) 86549..87121 - 573 WP_044266753.1 hypothetical protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / blaSHV-12 / blaOXA-10 / qacE / sul1 - 1..192902 192902


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(45-106)

Antitoxin

(39-83)


Sequences


Toxin        


Download         Length: 108 a.a.        Molecular weight: 12372.29 Da        Isoelectric Point: 9.6240

>T150026 WP_032720638.1 NZ_CP048801:81640-81963 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK

Download         Length: 324 bp

>T150026 NZ_CP064376:2827831-2828109 [Shigella sonnei]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11211.12 Da        Isoelectric Point: 7.3611

>AT150026 WP_032720637.1 NZ_CP048801:82012-82314 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV

Download         Length: 303 bp

>AT150026 NZ_CP064376:2827568-2827828 [Shigella sonnei]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCTGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A5Q9LMS5


Antitoxin

Source ID Structure

References