Detailed information of TA system TA150006

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 13599..14256
• Replicon: plasmid p8538-NDM-1
• Accession: NZ_CP048797
• Organism: Providencia vermicola strain P8538

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U3PDC3
• Locus tag: G3341_RS19630
• Protein ID: WP_000270043.1
• Coordinates: 13906..14256 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G3341_RS19625
• Protein ID: WP_000124640.1
• Coordinates: 13599..13901 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3341_RS19575	9233..9661	+	429	WP_000591076.1	hypothetical protein	-
G3341_RS19580	9719..10078	+	360	WP_000422769.1	hypothetical protein	-
G3341_RS19585	10078..10524	+	447	WP_000919343.1	hypothetical protein	-
G3341_RS19590	10521..11039	+	519	WP_000210757.1	nitrite reductase	-
G3341_RS19595	11039..11269	+	231	WP_000972665.1	hypothetical protein	-
G3341_RS19600	11256..12113	+	858	WP_001167036.1	hypothetical protein	-
G3341_RS20570	12025..12330	+	306	WP_171820680.1	hypothetical protein	-
G3341_RS19610	12333..12860	+	528	WP_004201083.1	thermonuclease family protein	-
G3341_RS19615	12918..13190	+	273	WP_001043046.1	HU family DNA-binding protein	-
G3341_RS19620	13279..13572	+	294	WP_001239998.1	hypothetical protein	-
G3341_RS19625	13599..13901	-	303	WP_000124640.1	XRE family transcriptional regulator	Antitoxin
G3341_RS19630	13906..14256	-	351	WP_000270043.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G3341_RS19635	14419..14967	+	549	WP_001061573.1	hypothetical protein	-
G3341_RS19645	15308..15502	+	195	WP_000343597.1	hypothetical protein	-
G3341_RS19650	15513..15884	+	372	WP_000516918.1	hypothetical protein	-
G3341_RS19655	15877..16347	+	471	WP_001281821.1	hypothetical protein	-
G3341_RS19660	16362..16697	-	336	WP_000683477.1	hypothetical protein	-
G3341_RS19665	16794..17282	+	489	WP_001273096.1	DUF1643 domain-containing protein	-
G3341_RS19670	17285..17782	+	498	WP_000062185.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaCMY-6 / aac(6')-Ib / qacE / sul1 / rmtC / blaNDM-1	htpB	1..151684	151684

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-106)

Antitoxin

• Cro/C1-type helix-turn-helix domain (33-79)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13328.21 Da
• Isoelectric Point: 5.2421

>T150006 WP_000270043.1 NZ_CP048797:c14256-13906 [Providencia vermicola]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Nucleotide

• Length: 351 bp

>T150006 NZ_CP064374:c689279-688938 [Enterococcus faecalis]
ATGAATGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGCAAAGAGAT
TCAGAAGAGACGGCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
CTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGGCAAGTAGTGATCTCACAA
CTGAAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11006.73 Da
• Isoelectric Point: 7.2036

>AT150006 WP_000124640.1 NZ_CP048797:c13901-13599 [Providencia vermicola]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Nucleotide

• Length: 303 bp

>AT150006 NZ_CP064374:c689509-689279 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAACTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GTCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Toxin

• AlphaFold DB: A0A7G8AFB9