Detailed information of TA system TA149999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1802974..1803706
• Replicon: chromosome
• Accession: NZ_CP048792
• Organism: Pasteurella multocida strain VP161

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: PmVP161_RS08275
• Protein ID: WP_061406326.1
• Coordinates: 1802974..1803300 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: PmVP161_RS08280
• Protein ID: WP_005757699.1
• Coordinates: 1803380..1803706 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PmVP161_RS08250	1798291..1798977	+	687	WP_061406323.1	hypothetical protein	-
PmVP161_RS08255	1799031..1800560	-	1530	WP_061406324.1	YifB family Mg chelatase-like AAA ATPase	-
PmVP161_RS08260	1800669..1801286	-	618	WP_005724253.1	ribosome biogenesis GTP-binding protein YihA/YsxC	-
PmVP161_RS08265	1801397..1802266	+	870	WP_061406325.1	VirK/YbjX family protein	-
PmVP161_RS08270	1802340..1802816	-	477	WP_042742721.1	tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL	-
PmVP161_RS08275	1802974..1803300	+	327	WP_061406326.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PmVP161_RS08280	1803380..1803706	+	327	WP_005757699.1	HigA family addiction module antidote protein	Antitoxin
PmVP161_RS08285	1803761..1805281	-	1521	WP_061406327.1	DUF560 domain-containing protein	-
PmVP161_RS08290	1805340..1805930	-	591	WP_061406438.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
PmVP161_RS08295	1805993..1807003	-	1011	WP_061406328.1	hypothetical protein	-
PmVP161_RS08300	1807239..1808270	-	1032	WP_061406329.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (12-104)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-102)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 12923.75 Da
• Isoelectric Point: 9.6705

>T149999 WP_061406326.1 NZ_CP048792:1802974-1803300 [Pasteurella multocida]
MDRVRFNLTEDSFQDKYLYEYFLYGSKHKNIPSDIENTLAHKLDMLDSANTLSDLRSQPGNKLEKLEPKTSGLYSIRVNV
KYRLIFKYKESNFPKITELRLDKHQYRL

Nucleotide

• Length: 327 bp

>T149999 NZ_CP064371:12272-12577 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACAGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12377.41 Da
• Isoelectric Point: 7.2034

>AT149999 WP_005757699.1 NZ_CP048792:1803380-1803706 [Pasteurella multocida]
MSRVQRKPSTVGDILLDEYLVPLNLKIADLATMLDVHRNTASALVNNNTKLSLEMALKLAKLFNTSPEFWLNLQMKLDLW
EIENNARFQESLKKISSIDQHNLLEKIA

Nucleotide

• Length: 327 bp

>AT149999 NZ_CP064371:12052-12270 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGTCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA