Detailed information of TA system TA149994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 3374075..3374673
• Replicon: chromosome
• Accession: NZ_CP048787
• Organism: Proteus mirabilis strain CC15031

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7Z7VLH5
• Locus tag: G4D35_RS15825
• Protein ID: WP_054103799.1
• Coordinates: 3374075..3374398 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G4D35_RS15830
• Protein ID: WP_000058216.1
• Coordinates: 3374395..3374673 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D35_RS15795	3369788..3370174	-	387	WP_032469918.1	hypothetical protein	-
G4D35_RS15800	3370171..3370743	-	573	WP_024008834.1	type IV conjugative transfer system lipoprotein TraV	-
G4D35_RS15805	3370818..3372107	-	1290	WP_064497041.1	TraB/VirB10 family protein	-
G4D35_RS15810	3372110..3373006	-	897	WP_064497055.1	type-F conjugative transfer system secretin TraK	-
G4D35_RS15815	3372990..3373616	-	627	WP_064497042.1	pilus assembly protein	-
G4D35_RS15820	3373613..3373894	-	282	WP_163801777.1	type IV conjugative transfer system protein TraL	-
G4D35_RS15825	3374075..3374398	+	324	WP_054103799.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4D35_RS15830	3374395..3374673	+	279	WP_000058216.1	helix-turn-helix domain-containing protein	Antitoxin
G4D35_RS15835	3374699..3375334	-	636	WP_000033756.1	DUF4400 domain-containing protein	-
G4D35_RS15840	3375321..3375881	-	561	WP_159262615.1	conjugative transfer protein	-
G4D35_RS15845	3375891..3377711	-	1821	WP_163801780.1	conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(J) / aadA2 / ere(A) / sul1 / qacE / ARR-3 / catB3 / blaOXA-1 / aac(6')-Ib-cr / aac(3)-IVa / aph(4)-Ia / sul2 / floR / blaCTX-M-65 / aph(3')-Ia / tet(C) / mph(E) / msr(E)	-	3223702..3441147	217445

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (15-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-81)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12295.35 Da
• Isoelectric Point: 9.7658

>T149994 WP_054103799.1 NZ_CP048787:3374075-3374398 [Proteus mirabilis]
MSWKIDFYDGVEEQILDMPPKIQARMIKLLELMEKHGANLGPPHTESMGDGLFEIRAKAQEGIGRGLFCYLKGKHIYVLH
AFVKKSQKTPKNELNLARDRQKEVQKS

Nucleotide

• Length: 324 bp

>T149994 NZ_CP064368:3986820-3987230 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10517.07 Da
• Isoelectric Point: 9.9393

>AT149994 WP_000058216.1 NZ_CP048787:3374395-3374673 [Proteus mirabilis]
MSLQKLKQRALANAEVKNEYDKLESEFSFIDQLLTMRTKAGLTQEQVAERMHTQKSNVSRLEKGNANPSWSTLLKYAHAC
GFELTLKPQKMS

Nucleotide

• Length: 279 bp

>AT149994 NZ_CP064368:3986573-3986839 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A7Z7VLH5