Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | higBA (relBE)/RelE-HTH |
| Location | 42306..42980 | Replicon | plasmid pEGKP7.2 |
| Accession | NZ_CP048780 | ||
| Organism | Klebsiella pneumoniae strain EGKP7 | ||
Toxin (Protein)
| Gene name | higB | Uniprot ID | A0A5Q9LMS5 |
| Locus tag | G4D19_RS27425 | Protein ID | WP_032720638.1 |
| Coordinates | 42306..42629 (+) | Length | 108 a.a. |
Antitoxin (Protein)
| Gene name | higA | Uniprot ID | - |
| Locus tag | G4D19_RS27430 | Protein ID | WP_032720637.1 |
| Coordinates | 42678..42980 (+) | Length | 101 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| G4D19_RS27385 (G4D19_27860) | 37575..37940 | - | 366 | WP_001294656.1 | mercuric ion transporter MerT | - |
| G4D19_RS27390 (G4D19_27865) | 38012..38467 | + | 456 | WP_001166628.1 | Hg(II)-responsive transcriptional regulator | - |
| G4D19_RS27395 (G4D19_27870) | 38501..39049 | + | 549 | Protein_48 | SOS response-associated peptidase family protein | - |
| G4D19_RS27400 (G4D19_27875) | 39238..39411 | + | 174 | WP_153591447.1 | hypothetical protein | - |
| G4D19_RS27405 (G4D19_27880) | 39445..40142 | + | 698 | WP_223367361.1 | IS1 family transposase | - |
| G4D19_RS27410 (G4D19_27885) | 40647..41249 | + | 603 | Protein_51 | RNA-guided endonuclease TnpB family protein | - |
| G4D19_RS27415 | 41318..41745 | - | 428 | Protein_52 | DNA-binding protein | - |
| G4D19_RS27420 (G4D19_27890) | 41723..42128 | + | 406 | Protein_53 | DUF4113 domain-containing protein | - |
| G4D19_RS27425 (G4D19_27895) | 42306..42629 | + | 324 | WP_032720638.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| G4D19_RS27430 (G4D19_27900) | 42678..42980 | + | 303 | WP_032720637.1 | NadS family protein | Antitoxin |
| G4D19_RS27435 (G4D19_27905) | 43023..43190 | - | 168 | WP_153591440.1 | hypothetical protein | - |
| G4D19_RS27440 (G4D19_27910) | 43378..43584 | - | 207 | WP_153591439.1 | hypothetical protein | - |
| G4D19_RS27445 (G4D19_27915) | 43581..43964 | - | 384 | WP_032720636.1 | hypothetical protein | - |
| G4D19_RS27450 (G4D19_27920) | 44069..44641 | - | 573 | WP_032720635.1 | GNAT family N-acetyltransferase | - |
| G4D19_RS27455 (G4D19_27925) | 44643..45455 | - | 813 | WP_223203129.1 | TSUP family transporter | - |
| G4D19_RS27460 (G4D19_27930) | 45467..46387 | - | 921 | WP_050548604.1 | TauD/TfdA family dioxygenase | - |
| G4D19_RS27465 (G4D19_27935) | 46690..47184 | - | 495 | WP_044266755.1 | hypothetical protein | - |
| G4D19_RS27470 (G4D19_27940) | 47215..47787 | - | 573 | WP_044266753.1 | hypothetical protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | blaNDM-1 / aph(3')-VI / qnrS1 / blaSHV-12 / aac(6')-Il / blaOXA-10 / qacE / sul1 | - | 1..153286 | 153286 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 108 a.a. Molecular weight: 12372.29 Da Isoelectric Point: 9.6240
>T149973 WP_032720638.1 NZ_CP048780:42306-42629 [Klebsiella pneumoniae]
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK
MNYLEFVETSAFSGLRKELMDDDEFRELQTYLLEAHDRGDTISHTGGCRKIRWGRPGMGKRGGVRVIYYVRLASGRLYLL
LIYPKNAKDELSEKEKAVMKALTQQLK
Download Length: 324 bp
>T149973 NZ_CP064363:1451110-1451430 [Salmonella enterica subsp. arizonae]
ATGCATATTTCAACTGTACCGGCCACGGTGCCGGTTTCGCCACGCCTGTCGCCCGTTCAGGTCTGGCAGCAACTGTTAAC
GTACCTACTGGAACATCATTACGGTCTTACGCTCAACGACACACCATTCCATGATAATTCAGCCATCCAGGAACATATCG
AGGCGGGTATCACACTTGCTGATGCGGTGAATTTCCTGATGGAACGCTATGAACTGGTGCGCATCGACCGTAAAGGATTC
ACCTGGCAAGAGCAGACACCATTCCTGACCACTATCGATTTACTCAGAGCCAAGCGAGCTACCGGATTAATGCACACATA
A
ATGCATATTTCAACTGTACCGGCCACGGTGCCGGTTTCGCCACGCCTGTCGCCCGTTCAGGTCTGGCAGCAACTGTTAAC
GTACCTACTGGAACATCATTACGGTCTTACGCTCAACGACACACCATTCCATGATAATTCAGCCATCCAGGAACATATCG
AGGCGGGTATCACACTTGCTGATGCGGTGAATTTCCTGATGGAACGCTATGAACTGGTGCGCATCGACCGTAAAGGATTC
ACCTGGCAAGAGCAGACACCATTCCTGACCACTATCGATTTACTCAGAGCCAAGCGAGCTACCGGATTAATGCACACATA
A
Antitoxin
Download Length: 101 a.a. Molecular weight: 11211.12 Da Isoelectric Point: 7.3611
>AT149973 WP_032720637.1 NZ_CP048780:42678-42980 [Klebsiella pneumoniae]
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV
MEKKLFDALISSMEEMVAIENGQIQPAAEQVHRHTLPDVKAIRKHAGMKQAEFAEVVGASVDLVRSWEQQRRVPSGIALK
MLCLIEREPALINTLRGIHV
Download Length: 303 bp
>AT149973 NZ_CP064363:1450711-1451076 [Salmonella enterica subsp. arizonae]
ATGCAATCGACCACCTTATCCGGCTCATCAGCGGAGAACGCATCCTGCCTTCAGTGGGGAATAAAACGTAATATCACGCC
GTGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCATCTCTACTTTCTGACTGATCGGTCTGGGTTTGATGGCTCATTCA
GTGACGATGAAGTATTGCGTCTGGACCAGGTGTTTCCACTGTTGATGAAACAGCTAGAGCGGATGCTCACCACTGGAGAG
CTTGATCCCCGCTGTCAACACTGCGTCACTCTGCATCACAATGGACTCACCTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATCGCTATTTATCCCCAGTCAGCCGTAACCAGGTAA
ATGCAATCGACCACCTTATCCGGCTCATCAGCGGAGAACGCATCCTGCCTTCAGTGGGGAATAAAACGTAATATCACGCC
GTGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCATCTCTACTTTCTGACTGATCGGTCTGGGTTTGATGGCTCATTCA
GTGACGATGAAGTATTGCGTCTGGACCAGGTGTTTCCACTGTTGATGAAACAGCTAGAGCGGATGCTCACCACTGGAGAG
CTTGATCCCCGCTGTCAACACTGCGTCACTCTGCATCACAATGGACTCACCTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATCGCTATTTATCCCCAGTCAGCCGTAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|