Detailed information of TA system TA149949

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 720290..720968
• Replicon: chromosome
• Accession: NZ_CP048751
• Organism: Brevundimonas mediterranea strain D151-2-6

Toxin (Protein)

• Gene name: higB
• Uniprot ID: B4WA80
• Locus tag: GYM46_RS03585
• Protein ID: WP_008259953.1
• Coordinates: 720290..720646 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GYM46_RS03590
• Protein ID: WP_008264199.1
• Coordinates: 720648..720968 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GYM46_RS03565	715400..715903	+	504	WP_035308435.1	hypothetical protein	-
GYM46_RS03570	716001..717389	+	1389	WP_008261181.1	cytochrome P450	-
GYM46_RS03575	717548..718567	+	1020	WP_008259271.1	glycosyltransferase family 4 protein	-
GYM46_RS03580	718666..720231	+	1566	WP_040349272.1	alkaline phosphatase D family protein	-
GYM46_RS03585	720290..720646	+	357	WP_008259953.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GYM46_RS03590	720648..720968	+	321	WP_008264199.1	XRE family transcriptional regulator	Antitoxin
GYM46_RS03595	721018..721839	-	822	WP_008261349.1	YmdB family metallophosphoesterase	-
GYM46_RS03600	721836..722255	-	420	WP_154726295.1	type II toxin-antitoxin system VapC family toxin	-
GYM46_RS03605	722252..722473	-	222	WP_196066381.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GYM46_RS03610	722517..723074	-	558	WP_008264368.1	5-formyltetrahydrofolate cyclo-ligase	-
GYM46_RS03620	723466..723786	-	321	WP_008263136.1	cell division protein ZapA	-
GYM46_RS03625	723786..724064	-	279	WP_008263869.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-108)

Antitoxin

• HigA2-like, helix-turn-helix domain (8-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13022.91 Da
• Isoelectric Point: 6.8809

>T149949 WP_008259953.1 NZ_CP048751:720290-720646 [Brevundimonas mediterranea]
MPWAVSNHDDFDPEFDALPVEVQDELLAMTALLEIYGPGLGRPHADTLTGSRHANMKELRFKAADGVWRVAFAFDPARQA
ILLVAGDKAGVAQKRFYKGLIAKADSRFTSHLKALKET

Nucleotide

• Length: 357 bp

>T149949 NZ_CP064352:c3428512-3427979 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 11645.49 Da
• Isoelectric Point: 8.7017

>AT149949 WP_008264199.1 NZ_CP048751:720648-720968 [Brevundimonas mediterranea]
MGRTREEIMAALPEARRARINARAAELVGEVEGLKALRELAERSQEQIAQALGVKQPSVLKMERQADLYLSTLRRFVEAA
GGTLELRVELPGKGVLHLTGMGDITH

Nucleotide

• Length: 321 bp

>AT149949 NZ_CP064352:c3428789-3428523 [Klebsiella pneumoniae subsp. pneumoniae ATCC 43816]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Toxin

• AlphaFold DB: B4WA80