Detailed information of TA system TA149874

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 40930..41515
• Replicon: plasmid pYH12138-1
• Accession: NZ_CP048671
• Organism: Acinetobacter sp. YH12138 strain YH12138_T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3F3L0E6
• Locus tag: G0029_RS16370
• Protein ID: WP_002126330.1
• Coordinates: 41195..41515 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G0029_RS16365
• Protein ID: WP_004676016.1
• Coordinates: 40930..41202 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0029_RS16340	36150..37097	-	948	WP_180109901.1	IS5 family transposase	-
G0029_RS16345	37566..37859	-	294	Protein_44	type II toxin-antitoxin system RelE/ParE family toxin	-
G0029_RS16350	37849..38094	-	246	WP_111885987.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
G0029_RS16355	38208..39542	-	1335	WP_180109902.1	ISNCY family transposase	-
G0029_RS16360	39808..40704	+	897	WP_180109903.1	sel1 repeat family protein	-
G0029_RS16365	40930..41202	-	273	WP_004676016.1	helix-turn-helix domain-containing protein	Antitoxin
G0029_RS16370	41195..41515	-	321	WP_002126330.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0029_RS16375	41733..42434	-	702	WP_180109904.1	DUF1851 domain-containing protein	-
G0029_RS16380	42519..43220	-	702	WP_180109905.1	DUF1851 domain-containing protein	-
G0029_RS16385	43315..43695	-	381	WP_180109906.1	hypothetical protein	-
G0029_RS16390	43697..43855	+	159	WP_180109907.1	hypothetical protein	-
G0029_RS16395	43878..45113	+	1236	WP_180109908.1	DUF3396 domain-containing protein	-
G0029_RS16400	45304..45854	-	551	Protein_55	hypothetical protein	-
G0029_RS16405	45904..46095	-	192	Protein_56	replication initiation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207449	207449
	-	inside	IScluster/Tn	-	-	28733..39542	10809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12191.98 Da
• Isoelectric Point: 7.9834

>T149874 WP_002126330.1 NZ_CP048671:c41515-41195 [Acinetobacter sp. YH12138]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVNEDDQIFFLVAY
PKSVKDNLTDKETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T149874 NZ_CP064326:1361449-1361745 [Xylella fastidiosa subsp. multiplex]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCGGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10178.56 Da
• Isoelectric Point: 7.3200

>AT149874 WP_004676016.1 NZ_CP048671:c41202-40930 [Acinetobacter sp. YH12138]
MDNNLFDDLVASIKEAGAIKRKEIQASRTTELELPDIKEVREKTGLSQSEFAARLHISHRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT149874 NZ_CP064326:1361748-1362041 [Xylella fastidiosa subsp. multiplex]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACGACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A3F3L0E6