Detailed information of TA system TA149873

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 30498..31083
• Replicon: plasmid pYH12138-1
• Accession: NZ_CP048671
• Organism: Acinetobacter sp. YH12138 strain YH12138_T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: G0029_RS16300
• Protein ID: WP_180109894.1
• Coordinates: 30763..31083 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G0029_RS16295
• Protein ID: WP_000369781.1
• Coordinates: 30498..30770 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0029_RS16270	25719..26930	+	1212	WP_180110283.1	tryptophan synthase subunit beta	-
G0029_RS16275	26927..27712	+	786	WP_180110284.1	tryptophan synthase subunit alpha	-
G0029_RS16280	27867..28720	+	854	Protein_31	transposase	-
G0029_RS16285	28733..29967	+	1235	Protein_32	IS3 family transposase	-
G0029_RS16290	30146..30403	+	258	WP_180109893.1	hypothetical protein	-
G0029_RS16295	30498..30770	-	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
G0029_RS16300	30763..31083	-	321	WP_180109894.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0029_RS16305	31250..31510	+	261	WP_180109895.1	hypothetical protein	-
G0029_RS16310	31649..31963	+	315	WP_180109896.1	hypothetical protein	-
G0029_RS16315	32442..33767	-	1326	WP_180109897.1	ISNCY family transposase	-
G0029_RS16320	34101..34604	-	504	WP_180109898.1	hypothetical protein	-
G0029_RS16325	34605..35351	-	747	WP_180109899.1	hypothetical protein	-
G0029_RS16330	35342..35766	-	425	Protein_41	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..207449	207449
	-	inside	IScluster/Tn	-	-	28733..39542	10809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12207.94 Da
• Isoelectric Point: 5.8723

>T149873 WP_180109894.1 NZ_CP048671:c31083-30763 [Acinetobacter sp. YH12138]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDEETAILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T149873 NZ_CP064326:c1042568-1042083 [Xylella fastidiosa subsp. multiplex]
GTGAATATGCAGATTTCGACACCTCATTCACTCACGGCAGCGCATCGACTTGATGAATTCAATTGCGGTGAGCCATCGCT
GGATGATTGGCTTAAACGACGCGCACTGACCAACCATTTAAACGGGGCCAGCCGGACCTTCGTGGTTATCGATGCCAACC
AATACGTGCTTGGGTACTACGCTTTGGCAGCCGGGGCTGTAGCGCATCAAGAGGCCACCCGTGCTATCCGACGTAACATG
CCTGATCCAGTGCCGGTCATGGTGCTCGGAAGGCTGGCTGTGGATACTCGTGCTCAAGGCATCAAACTCGGTGCGGCCTT
GCTTAAAGATACGGTAACTCGTGTGCAGTCCATTGCAGAGAACGCCGGCGTCAGGGCATTGGTAGTCCATGCGTTGAACG
AGCGCGCAAAGCAATTCTACGAGTACTATGGTTTTCGAGCGTCACCAATGCACCTGATGACCTTGATGCTTCCGATTAAA
TTCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT149873 WP_000369781.1 NZ_CP048671:c30770-30498 [Acinetobacter sp. YH12138]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT149873 NZ_CP064326:c1042837-1042565 [Xylella fastidiosa subsp. multiplex]
ATGCGCGACGCTTCCATCAACTTACGTGCCATGCCTGAGCAGCGCGACTTGATTGACCAGGCTGCTAATCTATTAGGCAA
GAATCGCTCAGACTTCATGCTAGAAGCTGCGTGCGAGCGTGCGAAAGCAATTATCCTGGATCAAGTCTTTTTCAATCTGG
ACGAAGAGAGGTTCCGGCAATTCACTGTGTTGCTTAATGCGCCACAAGACACCAATCCAGGCCTTAATCGCCTGATGGCA
ATTAAGGCTCCGTGGAGCATCAGCAATACGTGA