Detailed information of TA system TA149863

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 63443..64094
• Replicon: plasmid pC15-2
• Accession: NZ_CP048656
• Organism: Acinetobacter indicus strain C15_T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9NM16
• Locus tag: G0027_RS16335
• Protein ID: WP_004668307.1
• Coordinates: 63443..63799 (+)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G0027_RS16340
• Protein ID: WP_001140620.1
• Coordinates: 63792..64094 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0027_RS16310	58865..59059	-	195	WP_044432560.1	hypothetical protein	-
G0027_RS16315	59085..59696	-	612	WP_044432563.1	recombinase family protein	-
G0027_RS16320	59900..61225	-	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS16325	61388..62266	-	879	WP_180192461.1	DMT family transporter	-
G0027_RS16330	62359..62904	+	546	WP_005176794.1	XRE family transcriptional regulator	-
G0027_RS16335	63443..63799	+	357	WP_004668307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0027_RS16340	63792..64094	+	303	WP_001140620.1	XRE family transcriptional regulator	Antitoxin
G0027_RS16345	64215..65366	+	1152	WP_096901158.1	IS4 family transposase	-
G0027_RS16350	65414..65575	-	162	Protein_51	IS6 family transposase	-
G0027_RS16355	65669..66061	-	393	WP_007484277.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
G0027_RS16360	66151..67431	+	1281	WP_004668302.1	cation transporter	-
G0027_RS16365	67718..68164	+	447	WP_180192460.1	ribbon-helix-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..68627	68627
	-	inside	IScluster/Tn	-	-	49274..65575	16301

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• HigA2-like, helix-turn-helix domain (14-89)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 13610.64 Da
• Isoelectric Point: 7.1651

>T149863 WP_004668307.1 NZ_CP048656:63443-63799 [Acinetobacter indicus]
MWTVITTDLFNQWLEQQDEPTQEKVLAALVVLQQQGPSLGRPLVDTVYESKFTNMKELRVQHRGRPLRAFFAFDPLRQAI
VLCIADKGGKKRFYKDMLDIADEQYQLHLTTLGDKSNG

Nucleotide

• Length: 357 bp

>T149863 NZ_CP064325:c472884-472630 [Xylella fastidiosa subsp. multiplex]
ATGAAGCGTAAGCACGCTAAAACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCGATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGGCTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11227.02 Da
• Isoelectric Point: 6.4759

>AT149863 WP_001140620.1 NZ_CP048656:63792-64094 [Acinetobacter indicus]
MAKTLQELLASRSPESQARIQKMADELLLENQLHLIREELEISQKELAETLGIKQPSLSAIENRGNDLKISTMKKYVEAM
GGKLRIDVELPTGKHIGFNV

Nucleotide

• Length: 303 bp

>AT149863 NZ_CP064325:c472633-472298 [Xylella fastidiosa subsp. multiplex]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGTGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAAGACAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Structures

Toxin

• AlphaFold DB: N9NM16