Detailed information of TA system TA149861

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 53004..53589
• Replicon: plasmid pC15-1
• Accession: NZ_CP048655
• Organism: Acinetobacter indicus strain C15_T

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: G0027_RS15825
• Protein ID: WP_000897307.1
• Coordinates: 53004..53324 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G0027_RS15830
• Protein ID: WP_000369781.1
• Coordinates: 53317..53589 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G0027_RS15800	48806..49108	+	303	WP_000286964.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G0027_RS15805	49109..49390	+	282	WP_000985609.1	putative addiction module antidote protein	-
G0027_RS15810	49481..50806	-	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS15815	50957..51376	-	420	WP_001180106.1	SMI1/KNR4 family protein	-
G0027_RS15820	51555..52880	+	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS15825	53004..53324	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G0027_RS15830	53317..53589	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
G0027_RS15835	53680..55005	-	1326	WP_179994539.1	ISNCY family transposase	-
G0027_RS15840	55570..57045	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
G0027_RS15845	57101..57985	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	msr(E) / mph(E) / blaOXA-58 / aac(3)-IId / dfrA16 / blaCARB-2 / aadA2	-	1..104003	104003
	-	inside	IScluster/Tn	msr(E) / mph(E)	-	46070..64787	18717

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T149861 WP_000897307.1 NZ_CP048655:53004-53324 [Acinetobacter indicus]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T149861 NZ_CP064325:460330-460611 [Xylella fastidiosa subsp. multiplex]
ATGATCAAAAGTTTCCGCCACAAAGGCATTCAACAATTCTTCCTTGTGGGATCAACCGCTGGTATCCAAACCAAACACGC
CGCCAAACTGCGTATCCAGCTCACCGCGTTGGAGAGTGCCAAGCGCCCCGAAGATATGAATGCTCCAGGCTGGAAACTGC
ACCCGCTAAAAGGCGCTGACTTAAAAGGTCATTGGTCCATCTGGGTGAATGGCAACTATCGCCTCACGTTTGCTTTTGAA
GGCGAAGACGCAATCTTGGTCGACTATCAGGATTACCACTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT149861 WP_000369781.1 NZ_CP048655:53317-53589 [Acinetobacter indicus]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT149861 NZ_CP064325:459974-460273 [Xylella fastidiosa subsp. multiplex]
ATGACTATGTCTAAGACACTTCCCCCCATTCACCCAGGAGAAATTCTCCGGGAGGAGTTTATGGTCCCTCTAGGTCTGTC
CAGTAATGCGCTGGCTAAGGCCATTGGGGTGACGGCGGCACGCATCAATGAGATTGTGCGCGAGCGGCGCGGGATCACCG
CAGAGACTGCTTTGCGTCTGGCGCGCTACTTCGGTACCGATCCGCAAAGCTGGCTGAATTTGGAACAGCACTATGCGTTG
GAATGTGCCAAACGTGATCTTGGAGATGCGCTGTCACGGATTCATCCGCGAGTGGCTTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3