Detailed information of TA system TA149835

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 4199993..4200402
• Replicon: chromosome
• Accession: NZ_CP048647
• Organism: Escherichia coli strain GW-AmxH19

Toxin (Protein)

• Gene name: symE
• Uniprot ID: -
• Locus tag: G3273_RS20095
• Protein ID: WP_001534835.1
• Coordinates: 4200064..4200402 (+)
• Length: 113 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	4199993..4200069 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3273_RS20085	4195181..4196491	+	1311	WP_001534837.1	restriction endonuclease subunit S	-
G3273_RS20090	4196586..4199849	+	3264	WP_042050608.1	type I restriction endonuclease subunit R	-
-	4199993..4200069	-	77	NuclAT_12	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_12	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_12	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_12	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_13	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_13	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_13	-	Antitoxin
-	4199993..4200069	-	77	NuclAT_13	-	Antitoxin
G3273_RS20095	4200064..4200402	+	339	WP_001534835.1	endoribonuclease SymE	Toxin
G3273_RS20100	4200444..4200608	-	165	Protein_3943	DUF1127 domain-containing protein	-
G3273_RS20105	4200786..4200956	+	171	WP_000199350.1	GntR family transcriptional regulator	-
G3273_RS20110	4201086..4202006	-	921	WP_000181193.1	Rpn family recombination-promoting nuclease/putative transposase	-
G3273_RS20115	4202191..4203471	+	1281	WP_001535800.1	DUF445 domain-containing protein	-
G3273_RS20120	4203587..4204738	+	1152	WP_001338076.1	2-hydroxyacyl-CoA dehydratase subunit D	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12310.04 Da
• Isoelectric Point: 7.8226

>T149835 WP_001534835.1 NZ_CP048647:4200064-4200402 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYNRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAEESE
LMQSLRQVCKLSARKQKQVQDFIGVISNKTPR

Nucleotide

• Length: 339 bp

>T149835 NZ_CP064259:c342093-341623 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT149835 NZ_CP048647:c4200069-4199993 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin