Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1867273..1867775 Replicon chromosome
Accession NZ_CP048647
Organism Escherichia coli strain GW-AmxH19

Toxin (Protein)


Gene name yoeB Uniprot ID D8A584
Locus tag G3273_RS08750 Protein ID WP_000767828.1
Coordinates 1867521..1867775 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag G3273_RS08745 Protein ID WP_001259255.1
Coordinates 1867273..1867524 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3273_RS08720 1862451..1863518 - 1068 WP_000080129.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
G3273_RS08725 1863518..1864588 - 1071 WP_000108949.1 histidinol-phosphate transaminase -
G3273_RS08730 1864585..1865889 - 1305 WP_001370829.1 histidinol dehydrogenase -
G3273_RS08735 1865895..1866794 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
G3273_RS08740 1866940..1866990 - 51 WP_001364200.1 his operon leader peptide -
G3273_RS08745 1867273..1867524 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
G3273_RS08750 1867521..1867775 + 255 WP_000767828.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
G3273_RS08755 1867858..1868682 + 825 WP_000754740.1 SDR family oxidoreductase -
G3273_RS08760 1868728..1869657 + 930 WP_000803351.1 LysR family transcriptional regulator -
G3273_RS08765 1869872..1869934 + 63 WP_010723108.1 membrane protein YoeI -
G3273_RS08770 1869924..1871282 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
G3273_RS08775 1871461..1872519 + 1059 WP_000492324.1 transport protein YeeE -
G3273_RS08780 1872533..1872760 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10169.56 Da        Isoelectric Point: 8.0353

>T149817 WP_000767828.1 NZ_CP048647:1867521-1867775 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEDHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T149817 NZ_CP064253:c64373-64215 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT149817 WP_001259255.1 NZ_CP048647:1867273-1867524 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT149817 NZ_CP064253:c64641-64417 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G465

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References