Detailed information of TA system TA149816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1176432..1177159
• Replicon: chromosome
• Accession: NZ_CP048647
• Organism: Escherichia coli strain GW-AmxH19

Toxin (Protein)

• Gene name: higB
• Uniprot ID: Q3YYE6
• Locus tag: G3273_RS05600
• Protein ID: WP_000547563.1
• Coordinates: 1176432..1176743 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G3273_RS05605
• Protein ID: WP_000126304.1
• Coordinates: 1176740..1177159 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3273_RS05570	1171576..1173285	+	1710	WP_001288123.1	formate hydrogenlyase subunit HycE	-
G3273_RS05575	1173295..1173837	+	543	WP_000493800.1	formate hydrogenlyase subunit HycF	-
G3273_RS05580	1173837..1174604	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
G3273_RS05585	1174601..1175011	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
G3273_RS05590	1175004..1175471	+	468	WP_000132955.1	hydrogenase maturation peptidase HycI	-
G3273_RS05595	1175514..1176269	+	756	WP_001314074.1	hypothetical protein	-
G3273_RS05600	1176432..1176743	+	312	WP_000547563.1	type II toxin-antitoxin system HigB family toxin	Toxin
G3273_RS05605	1176740..1177159	+	420	WP_000126304.1	helix-turn-helix domain-containing protein	Antitoxin
G3273_RS05610	1177251..1177679	-	429	WP_000536066.1	DUF4259 domain-containing protein	-
G3273_RS05615	1178064..1178591	+	528	WP_001078777.1	electron transport protein HydN	-
G3273_RS05620	1178744..1180996	+	2253	WP_001535518.1	carbamoyltransferase HypF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1147586..1186504	38918

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12398.11 Da
• Isoelectric Point: 9.7105

>T149816 WP_000547563.1 NZ_CP048647:1176432-1176743 [Escherichia coli]
MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T149816 NZ_CP064253:11314-11730 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15474.50 Da
• Isoelectric Point: 4.4596

>AT149816 WP_000126304.1 NZ_CP048647:1176740-1177159 [Escherichia coli]
MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT149816 NZ_CP064253:11087-11317 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9QA22