Detailed information of TA system TA149789

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 3144..3558
• Replicon: chromosome
• Accession: NZ_CP048643
• Organism: Staphylococcus aureus strain SR153

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GAU34_RS00030
• Protein ID: WP_031267622.1
• Coordinates: 3307..3558 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GAU34_RS14735
• Protein ID: WP_000028424.1
• Coordinates: 3144..3317 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GAU34_RS00010	1774..1974	-	201	WP_000265252.1	DUF1514 family protein	-
GAU34_RS00015	2042..2194	-	153	WP_000595263.1	transcriptional activator RinB	-
GAU34_RS00020	2191..2397	-	207	WP_000195810.1	DUF1381 domain-containing protein	-
GAU34_RS00025	2434..2967	-	534	WP_063653166.1	dUTP diphosphatase	-
GAU34_RS14730	2982..3143	-	162	WP_000889684.1	hypothetical protein	-
GAU34_RS14735	3144..3317	-	174	WP_000028424.1	hypothetical protein	Antitoxin
GAU34_RS00030	3307..3558	-	252	WP_031267622.1	DUF1024 family protein	Toxin
GAU34_RS00035	3551..3857	-	307	Protein_8	hypothetical protein	-
GAU34_RS00040	3854..4201	-	348	WP_000979209.1	hypothetical protein	-
GAU34_RS00045	4198..4578	-	381	WP_001668982.1	hypothetical protein	-
GAU34_RS00050	4581..4787	-	207	WP_000693989.1	hypothetical protein	-
GAU34_RS00055	4801..5049	-	249	WP_060474161.1	hypothetical protein	-
GAU34_RS00060	5049..5303	-	255	WP_000022722.1	DUF3310 domain-containing protein	-
GAU34_RS00065	5300..5704	-	405	WP_044426014.1	phage DNA polymerase	-
GAU34_RS00070	5704..5889	-	186	WP_001164629.1	DUF3113 family protein	-
GAU34_RS00075	5902..7854	-	1953	WP_161081393.1	DNA polymerase	-
GAU34_RS00080	7923..8480	-	558	WP_000645042.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1774..17661	15887

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9419.35 Da
• Isoelectric Point: 3.9476

>T149789 WP_031267622.1 NZ_CP048643:c3558-3307 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T149789 NZ_CP064246:357447-357794 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT149789 WP_000028424.1 NZ_CP048643:c3317-3144 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT149789 NZ_CP064246:357794-358093 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA