Detailed information of TA system TA149759

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 63538..63792
• Replicon: plasmid unnamed1
• Accession: NZ_CP048610
• Organism: Escherichia coli strain STEFF_1

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: G3M66_RS24395
• Protein ID: WP_001312851.1
• Coordinates: 63643..63792 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	63538..63599 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M66_RS24360 (59190)	59190..59402	+	213	WP_005012601.1	hypothetical protein	-
G3M66_RS24365 (59703)	59703..59792	-	90	Protein_63	IS1 family transposase	-
G3M66_RS24370 (59847)	59847..60524	+	678	WP_001339397.1	IS66-like element accessory protein TnpA	-
G3M66_RS24375 (60524)	60524..60871	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
G3M66_RS24380 (60891)	60891..62462	+	1572	WP_023149734.1	IS66-like element ISCro1 family transposase	-
G3M66_RS24385 (62500)	62500..63116	-	617	Protein_67	IS1-like element IS1A family transposase	-
G3M66_RS24390 (63217)	63217..63399	+	183	WP_000968309.1	hypothetical protein	-
- (63538)	63538..63599	-	62	NuclAT_0	-	Antitoxin
- (63538)	63538..63599	-	62	NuclAT_0	-	Antitoxin
- (63538)	63538..63599	-	62	NuclAT_0	-	Antitoxin
- (63538)	63538..63599	-	62	NuclAT_0	-	Antitoxin
G3M66_RS24395 (63643)	63643..63792	+	150	WP_001312851.1	Hok/Gef family protein	Toxin
G3M66_RS24400 (64076)	64076..64333	+	258	WP_000083833.1	replication regulatory protein RepA	-
G3M66_RS24405 (64569)	64569..64643	+	75	WP_032336874.1	RepA leader peptide Tap	-
G3M66_RS24410 (64636)	64636..65082	+	447	Protein_72	plasmid replication initiator RepA	-
G3M66_RS24415 (65082)	65082..65696	-	615	Protein_73	VENN motif pre-toxin domain-containing protein	-
G3M66_RS24420 (66403)	66403..67623	+	1221	WP_000410951.1	arginine deiminase	-
G3M66_RS24425 (67634)	67634..68545	+	912	WP_000440183.1	carbamate kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(M) / ant(3'')-Ia / cmlA1 / aadA2 / dfrA12 / floR / sul2 / tet(A) / sul3 / blaTEM-1B / aph(3')-Ia / aac(3)-IId / sitABCD	iutA / iucD / iucC / iucB / iucA	1..119126	119126

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T149759 WP_001312851.1 NZ_CP048610:63643-63792 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T149759 NZ_CP064236:c111276-110965 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT149759 NZ_CP048610:c63599-63538 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin