Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2412895..2413457 Replicon chromosome
Accession NZ_CP048609
Organism Escherichia coli strain STEFF_1

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag G3M66_RS11600 Protein ID WP_000605676.1
Coordinates 2413179..2413457 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag G3M66_RS11595 Protein ID WP_000781370.1
Coordinates 2412895..2413179 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M66_RS11580 (2408254) 2408254..2411301 + 3048 WP_012311994.1 formate dehydrogenase-N subunit alpha -
G3M66_RS11585 (2411314) 2411314..2412198 + 885 WP_001240582.1 formate dehydrogenase N subunit beta -
G3M66_RS11590 (2412191) 2412191..2412844 + 654 WP_000045650.1 formate dehydrogenase-N subunit gamma -
G3M66_RS11595 (2412895) 2412895..2413179 - 285 WP_000781370.1 HigA family addiction module antitoxin Antitoxin
G3M66_RS11600 (2413179) 2413179..2413457 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
G3M66_RS11605 (2413643) 2413643..2414653 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
G3M66_RS11610 (2414787) 2414787..2416484 - 1698 WP_000433464.1 malate dehydrogenase -
G3M66_RS11615 (2416641) 2416641..2416778 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
G3M66_RS11620 (2416880) 2416880..2417095 - 216 WP_000495766.1 biofilm-dependent modulation protein -
G3M66_RS11625 (2417440) 2417440..2417871 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T149741 WP_000605676.1 NZ_CP048609:c2413457-2413179 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T149741 NZ_CP064232:c4856571-4856287 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT149741 WP_000781370.1 NZ_CP048609:c2413179-2412895 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT149741 NZ_CP064232:c4856803-4856561 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References