Detailed information of TA system TA149735

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 677677..678476
• Replicon: chromosome
• Accession: NZ_CP048609
• Organism: Escherichia coli strain STEFF_1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: G3M66_RS03295
• Protein ID: WP_000347273.1
• Coordinates: 677677..678141 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: G3M66_RS03300
• Protein ID: WP_001308975.1
• Coordinates: 678141..678476 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M66_RS03265 (672678)	672678..673112	-	435	WP_000948834.1	PTS galactosamine/N-acetylgalactosamine transporter subunit IIA	-
G3M66_RS03270 (673130)	673130..674008	-	879	WP_001298314.1	PTS N-acetylgalactosamine transporter subunit IID	-
G3M66_RS03275 (673998)	673998..674777	-	780	WP_000406214.1	PTS N-acetylgalactosamine transporter subunit IIC	-
G3M66_RS03280 (674788)	674788..675261	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
G3M66_RS03285 (675284)	675284..676564	-	1281	WP_000681961.1	tagatose-bisphosphate aldolase subunit KbaZ	-
G3M66_RS03290 (676813)	676813..677622	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
G3M66_RS03295 (677677)	677677..678141	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
G3M66_RS03300 (678141)	678141..678476	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
G3M66_RS03305 (678625)	678625..680196	-	1572	WP_001273756.1	galactarate dehydratase	-
G3M66_RS03310 (680571)	680571..681905	+	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
G3M66_RS03315 (681921)	681921..682691	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T149735 WP_000347273.1 NZ_CP048609:c678141-677677 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T149735 NZ_CP064232:732175-732660 [Klebsiella pneumoniae]
ATGATCTCGGCACCAGAACCGCTTCATGCCGGGCATATTCTTACCCCGTTTTGCTGCGGTATAGATTCCATGGATCACTG
GCTGAAACAGCGGGCGATGAAAAATCAGGTTACTGGCGCATCCCGCACCTTTGTCTGCTGCGACGACGCGAAGGTGATGG
CTTACTACTCACTGGCTTCCAGCGCTGTGACGACAAATACTGCGCCGGGTCGTTTTCGCCGCAATATGCCTGACCCGATC
CCGGTTGTGGTACTGGGTCGTCTGGCGGTGGATAAATCACTACATGGGAAAGGTGTCGGGCGGGCGCTGGTACGTGATGC
CGGGCTGCGTGTGATTCAGGTGGCGGAGACTATCGGCATTCGCGGGATGCTGGTTCACGCCCTGTCGGATGAAGCACGGG
ATTTTTACCTGCGGGTGGGGTTTGAGCCGTCGCCGATGGATTCGATGATGTTGATGGTGACGTTGAGGGATTTGGTTAAT
GCCTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT149735 WP_001308975.1 NZ_CP048609:c678476-678141 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT149735 NZ_CP064232:731885-732178 [Klebsiella pneumoniae]
ATGCCAGCCGCAAACAGCATGTCCATAAAACGCGAAACGTTAAATCTGCGCATTAAACCTGCCGAACGCGATCTTATTGA
TCGGGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAGGCCGCCCGCGCTGCCGCTGAGGAAGCGCTGA
TTGAGCAACGCATCATTATGGCCGATCCGCAGGCTTATCAGGAATTTCTCGCTCGTCTGGATCAGGCTCCTGCACCTAAT
GCCGCACTGCGTAAAACTATGCAGACCCCTGCGCCGTGGGAACAGGAAGAATGA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08