Detailed information of TA system TA149729

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1107606..1108210
• Replicon: chromosome
• Accession: NZ_CP048607
• Organism: Pseudomonas fluorescens strain DR133

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GZ982_RS04910
• Protein ID: WP_116831887.1
• Coordinates: 1107606..1107920 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A6N1CCA5
• Locus tag: GZ982_RS04915
• Protein ID: WP_058545613.1
• Coordinates: 1107923..1108210 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GZ982_RS04890	1103068..1104639	-	1572	WP_116831890.1	GGDEF domain-containing protein	-
GZ982_RS04895	1104851..1105510	-	660	WP_116831889.1	ribonuclease T2	-
GZ982_RS04900	1105689..1107047	+	1359	WP_109751620.1	chromate efflux transporter	-
GZ982_RS04905	1107044..1107475	-	432	WP_163854607.1	GNAT family N-acetyltransferase	-
GZ982_RS04910	1107606..1107920	+	315	WP_116831887.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GZ982_RS04915	1107923..1108210	+	288	WP_058545613.1	helix-turn-helix domain-containing protein	Antitoxin
GZ982_RS04920	1108354..1108839	+	486	WP_163854610.1	hypothetical protein	-
GZ982_RS04925	1108855..1109253	-	399	WP_204334273.1	winged helix-turn-helix transcriptional regulator	-
GZ982_RS04930	1109341..1109748	+	408	WP_163854612.1	DoxX family protein	-
GZ982_RS04935	1109760..1110977	-	1218	WP_163854615.1	MFS transporter	-
GZ982_RS04940	1111104..1112153	-	1050	WP_163854618.1	NAD(P)-dependent alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (35-86)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11934.03 Da
• Isoelectric Point: 10.1359

>T149729 WP_116831887.1 NZ_CP048607:1107606-1107920 [Pseudomonas fluorescens]
MIFIETPIFTRRVKELMDDDDFAVLQKILVCNPSAGDVMEATGGIRKIRIAAKGHGKRGGARVIYYHFVHASRIALLMIY
PKNEQQDLTVDQRKALKLIVEHWR

Nucleotide

• Length: 315 bp

>T149729 NZ_CP064230:c13754-13284 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10741.40 Da
• Isoelectric Point: 8.7885

>AT149729 WP_058545613.1 NZ_CP048607:1107923-1108210 [Pseudomonas fluorescens]
MSKFFDELMESVQQMDEIVRGERQPSREFIVDALQVKEIRKATGLTQAKFAALIDVQLGTLRNWEQGRREPTGPAKALLR
AIHNDPKHVISALAV

Nucleotide

• Length: 288 bp

>AT149729 NZ_CP064230:c14194-13751 [Klebsiella pneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAATCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTACGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACAGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6N1CCA5