Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 2038421..2038962 Replicon chromosome
Accession NZ_CP048605
Organism Escherichia coli strain PapRG-06-5

Toxin (Protein)


Gene name yafQ Uniprot ID Q32JN3
Locus tag G3M73_RS09605 Protein ID WP_000615980.1
Coordinates 2038684..2038962 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID Q32JN2
Locus tag G3M73_RS09600 Protein ID WP_000729709.1
Coordinates 2038421..2038681 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M73_RS09580 2034127..2034912 - 786 WP_163425180.1 putative lateral flagellar export/assembly protein LafU -
G3M73_RS09585 2034884..2036596 + 1713 Protein_1886 flagellar biosynthesis protein FlhA -
G3M73_RS09590 2036789..2037286 - 498 WP_163425181.1 transposase -
G3M73_RS09595 2037462..2038211 - 750 WP_024191992.1 C40 family peptidase -
G3M73_RS09600 2038421..2038681 + 261 WP_000729709.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G3M73_RS09605 2038684..2038962 + 279 WP_000615980.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G3M73_RS09610 2039118..2039858 + 741 WP_001225679.1 murein L,D-transpeptidase -
G3M73_RS09615 2039829..2040596 - 768 WP_000333379.1 class II glutamine amidotransferase -
G3M73_RS09620 2040802..2041380 - 579 WP_163425182.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA / hsiC1/vipB / hsiB1/vipA 1975070..2136507 161437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(5-92)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10830.62 Da        Isoelectric Point: 10.0702

>T149717 WP_000615980.1 NZ_CP048605:2038684-2038962 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAHVEPDWLLIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T149717 NZ_CP064226:c90837-90688 [Klebsiella pneumoniae]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9333.73 Da        Isoelectric Point: 5.8129

>AT149717 WP_000729709.1 NZ_CP048605:2038421-2038681 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVGVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT149717 NZ_CP064226:90882-90941 [Klebsiella pneumoniae]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090NH48

Antitoxin

Source ID Structure
AlphaFold DB A0A829FY17

References