Detailed information of TA system TA149716

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 2032255..2032949
• Replicon: chromosome
• Accession: NZ_CP048605
• Organism: Escherichia coli strain PapRG-06-5

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A080JBV5
• Locus tag: G3M73_RS09565
• Protein ID: WP_032280310.1
• Coordinates: 2032255..2032653 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G3M73_RS09570
• Protein ID: WP_000554758.1
• Coordinates: 2032656..2032949 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M73_RS09535	2027370..2028614	-	1245	WP_000189541.1	esterase FrsA	-
-	2028030..2028110	-	81	NuclAT_12	-	-
-	2028030..2028110	-	81	NuclAT_12	-	-
-	2028030..2028110	-	81	NuclAT_12	-	-
-	2028030..2028110	-	81	NuclAT_12	-	-
G3M73_RS09540	2028706..2029164	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
G3M73_RS09545	2029425..2030882	+	1458	WP_032187154.1	cytosol nonspecific dipeptidase	-
G3M73_RS09550	2030939..2031247	-	309	Protein_1879	peptide chain release factor H	-
G3M73_RS09555	2031267..2031475	-	209	Protein_1880	RtcB family protein	-
G3M73_RS09560	2031793..2032245	-	453	WP_001059871.1	GNAT family N-acetyltransferase	-
G3M73_RS09565	2032255..2032653	-	399	WP_032280310.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G3M73_RS09570	2032656..2032949	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G3M73_RS09575	2033001..2034056	-	1056	WP_001226183.1	DNA polymerase IV	-
G3M73_RS09580	2034127..2034912	-	786	WP_163425180.1	putative lateral flagellar export/assembly protein LafU	-
G3M73_RS09585	2034884..2036596	+	1713	Protein_1886	flagellar biosynthesis protein FlhA	-
G3M73_RS09590	2036789..2037286	-	498	WP_163425181.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE / gmhA/lpcA / hsiC1/vipB / hsiB1/vipA	1975070..2136507	161437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15501.91 Da
• Isoelectric Point: 8.0949

>T149716 WP_032280310.1 NZ_CP048605:c2032653-2032255 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTVDFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDESHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T149716 NZ_CP064226:c62154-61879 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT149716 WP_000554758.1 NZ_CP048605:c2032949-2032656 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT149716 NZ_CP064226:c62438-62154 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A080JBV5

Antitoxin

• AlphaFold DB: S1QAE3