Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3599311..3600110 Replicon chromosome
Accession NZ_CP048604
Organism Escherichia coli strain PapRG-06-3

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag G3M72_RS17160 Protein ID WP_000347266.1
Coordinates 3599311..3599775 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag G3M72_RS17165 Protein ID WP_001307405.1
Coordinates 3599775..3600110 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M72_RS17130 3594312..3594746 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
G3M72_RS17135 3594764..3595642 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
G3M72_RS17140 3595632..3596411 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
G3M72_RS17145 3596422..3596895 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
G3M72_RS17150 3596918..3598198 - 1281 WP_032296572.1 tagatose-bisphosphate aldolase subunit KbaZ -
G3M72_RS17155 3598447..3599256 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
G3M72_RS17160 3599311..3599775 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
G3M72_RS17165 3599775..3600110 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
G3M72_RS17170 3600259..3601830 - 1572 WP_040091028.1 galactarate dehydratase -
G3M72_RS17175 3602205..3603539 + 1335 WP_032296573.1 galactarate/glucarate/glycerate transporter GarP -
G3M72_RS17180 3603555..3604325 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3599311..3610872 11561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T149699 WP_000347266.1 NZ_CP048604:c3599775-3599311 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T149699 NZ_CP064223:329444-329812 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT149699 WP_001307405.1 NZ_CP048604:c3600110-3599775 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT149699 NZ_CP064223:329226-329447 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References