Detailed information of TA system TA149698

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2800141..2800743
• Replicon: chromosome
• Accession: NZ_CP048604
• Organism: Escherichia coli strain PapRG-06-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: G3M72_RS13295
• Protein ID: WP_000897305.1
• Coordinates: 2800432..2800743 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: G3M72_RS13290
• Protein ID: WP_000356397.1
• Coordinates: 2800141..2800431 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M72_RS13265	2796067..2796969	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
G3M72_RS13270	2796966..2797601	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
G3M72_RS13275	2797598..2798527	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
G3M72_RS13280	2798857..2799099	-	243	WP_001086388.1	hypothetical protein	-
G3M72_RS13285	2799318..2799536	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
G3M72_RS13290	2800141..2800431	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
G3M72_RS13295	2800432..2800743	-	312	WP_000897305.1	hypothetical protein	Toxin
G3M72_RS13300	2800972..2801880	+	909	WP_001162704.1	alpha/beta hydrolase	-
G3M72_RS13305	2801944..2802885	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
G3M72_RS13310	2802930..2803367	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
G3M72_RS13315	2803364..2804236	-	873	WP_000920762.1	virulence factor BrkB family protein	-
G3M72_RS13320	2804230..2804829	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T149698 WP_000897305.1 NZ_CP048604:c2800743-2800432 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T149698 NZ_CP064222:1604-1870 [Klebsiella pneumoniae]
TTGGTCTGGACGATTAACTATTCCGATCGGGCGCTCAAATCGTTACGCAAGATGGACAAACAGAACGCACGACGGATTGT
GGATTTTATGAGTTTACGCATTGCAGTTGCTGCCGATCCTCGCCAGTCAGGGAAGCCGCTCAAAGGTGAGCTGGGCGAGT
TCTGGCGCTATCGGGTGGGAGATTATCGCGTTCTGTGTGAGATCCGAGATGACGAGCTTGTTATCCTTGCCGCCACGATT
GGACATCGCCGCGAAGTTTACGACTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT149698 WP_000356397.1 NZ_CP048604:c2800431-2800141 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT149698 NZ_CP064222:1396-1620 [Klebsiella pneumoniae]
ATGCTTGCTATCCGGTTATCTGATGAGATTGAGTCCCGTCTGGACTCGCTGGCGAAGCAAACCGGCAGAACAAAGACGTT
TTATGCGCGGGAAGCAATACTGGCGCATCTGGAAGACCTGGAGGATTATTATCTTTCAGCAGAAACTGCTGCACGCGTTC
GCCGTGGTGATGAAGCAGTGCATTCGTCTGAAGACGTGAGGAAGTCACTTGGTCTGGACGATTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7