Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1985146..1985687 Replicon chromosome
Accession NZ_CP048604
Organism Escherichia coli strain PapRG-06-3

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag G3M72_RS09590 Protein ID WP_000615976.1
Coordinates 1985409..1985687 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag G3M72_RS09585 Protein ID WP_000729704.1
Coordinates 1985146..1985406 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
G3M72_RS09560 1980929..1981714 - 786 WP_000207575.1 putative lateral flagellar export/assembly protein LafU -
G3M72_RS09565 1981686..1983398 + 1713 Protein_1877 flagellar biosynthesis protein FlhA -
G3M72_RS09570 1983503..1983781 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
G3M72_RS09575 1983774..1984130 + 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
G3M72_RS09580 1984187..1984960 - 774 WP_000543895.1 C40 family peptidase -
G3M72_RS09585 1985146..1985406 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
G3M72_RS09590 1985409..1985687 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
G3M72_RS09595 1985843..1986583 + 741 WP_001225679.1 murein L,D-transpeptidase -
G3M72_RS09600 1986554..1987321 - 768 WP_000333380.1 class II glutamine amidotransferase -
G3M72_RS09605 1987527..1988105 - 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T149696 WP_000615976.1 NZ_CP048604:1985409-1985687 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T149696 NZ_CP064220:c61535-61260 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT149696 WP_000729704.1 NZ_CP048604:1985146-1985406 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT149696 NZ_CP064220:c61819-61535 [Klebsiella pneumoniae]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGGCTGGAACGCTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References