Detailed information of TA system TA149676

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symER/SymE(toxin)
• Location: 3963139..3963551
• Replicon: chromosome
• Accession: NZ_CP048603
• Organism: Escherichia coli strain ChlosBP-23-1

Toxin (Protein)

• Gene name: symE
• Uniprot ID: S1NWQ7
• Locus tag: G3M71_RS18970
• Protein ID: WP_000132614.1
• Coordinates: 3963210..3963551 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	symR
Locus tag	-
Coordinates	3963139..3963215 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M71_RS18960	3959739..3961208	+	1470	WP_204261137.1	N-6 DNA methylase	-
G3M71_RS18965	3961208..3962989	+	1782	WP_001765406.1	restriction endonuclease subunit S	-
-	3963139..3963215	-	77	NuclAT_6	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_6	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_6	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_6	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_7	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_7	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_7	-	Antitoxin
-	3963139..3963215	-	77	NuclAT_7	-	Antitoxin
G3M71_RS18970	3963210..3963551	+	342	WP_000132614.1	endoribonuclease SymE	Toxin
G3M71_RS18975	3963598..3965691	-	2094	WP_163388734.1	DUF262 domain-containing protein	-
G3M71_RS18980	3965789..3965869	-	81	WP_020233658.1	hypothetical protein	-
G3M71_RS18985	3966097..3967017	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
G3M71_RS18990	3967202..3968482	+	1281	WP_077561073.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	cheD	3946943..3963551	16608

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12266.07 Da
• Isoelectric Point: 8.4982

>T149676 WP_000132614.1 NZ_CP048603:3963210-3963551 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T149676 NZ_CP064214:c64242-63967 [Klebsiella pneumoniae]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAATCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT149676 NZ_CP048603:c3963215-3963139 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: S1NWQ7

Antitoxin