Detailed information of TA system TA149673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3628829..3629523
• Replicon: chromosome
• Accession: NZ_CP048603
• Organism: Escherichia coli strain ChlosBP-23-1

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: G3M71_RS17415
• Protein ID: WP_001263500.1
• Coordinates: 3628829..3629227 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: G3M71_RS17420
• Protein ID: WP_000554758.1
• Coordinates: 3629230..3629523 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G3M71_RS17390	3624194..3624652	-	459	WP_163388694.1	xanthine phosphoribosyltransferase	-
G3M71_RS17395	3624913..3626370	+	1458	WP_163388695.1	cytosol nonspecific dipeptidase	-
G3M71_RS17400	3626427..3627041	-	615	Protein_3403	peptide chain release factor H	-
G3M71_RS17405	3627038..3628177	-	1140	WP_000521561.1	RNA ligase RtcB family protein	-
G3M71_RS17410	3628367..3628819	-	453	WP_001059895.1	GNAT family N-acetyltransferase	-
G3M71_RS17415	3628829..3629227	-	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
G3M71_RS17420	3629230..3629523	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
G3M71_RS17425	3629575..3630630	-	1056	WP_001226168.1	DNA polymerase IV	-
G3M71_RS17430	3630701..3631486	-	786	WP_000207578.1	putative lateral flagellar export/assembly protein LafU	-
G3M71_RS17435	3631458..3633170	+	1713	Protein_3410	flagellar biosynthesis protein FlhA	-
G3M71_RS17440	3633247..3634020	-	774	WP_163388696.1	C40 family peptidase	-
G3M71_RS17445	3634206..3634466	+	261	WP_000729704.1	type II toxin-antitoxin system antitoxin DinJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T149673 WP_001263500.1 NZ_CP048603:c3629227-3628829 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T149673 NZ_CP064212:171932-172348 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT149673 WP_000554758.1 NZ_CP048603:c3629523-3629230 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT149673 NZ_CP064212:171705-171935 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3