Detailed information of TA system TA149627

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1857581..1858217
• Replicon: chromosome
• Accession: NZ_CP048585
• Organism: Agrobacterium pusense strain CC001

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: FY130_RS21995
• Protein ID: WP_262622535.1
• Coordinates: 1857581..1857862 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: FY130_RS22000
• Protein ID: WP_004444714.1
• Coordinates: 1857918..1858217 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FY130_RS21975 (FY130_21830)	1853616..1854623	+	1008	WP_262622532.1	iron-siderophore ABC transporter substrate-binding protein	-
FY130_RS21980 (FY130_21835)	1854620..1855663	+	1044	WP_262622533.1	iron ABC transporter permease	-
FY130_RS21985 (FY130_21840)	1855666..1856700	+	1035	WP_262622708.1	iron chelate uptake ABC transporter family permease subunit	-
FY130_RS21990 (FY130_21845)	1856697..1857503	+	807	WP_262622534.1	ABC transporter ATP-binding protein	-
FY130_RS21995 (FY130_21850)	1857581..1857862	+	282	WP_262622535.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FY130_RS22000 (FY130_21855)	1857918..1858217	+	300	WP_004444714.1	HigA family addiction module antitoxin	Antitoxin
FY130_RS22005 (FY130_21860)	1858237..1859109	-	873	WP_262622536.1	helix-turn-helix transcriptional regulator	-
FY130_RS22010 (FY130_21865)	1859242..1860456	+	1215	WP_262622537.1	amino acid ABC transporter substrate-binding protein	-
FY130_RS22015 (FY130_21870)	1860527..1861399	+	873	WP_262622539.1	branched-chain amino acid ABC transporter permease	-
FY130_RS22020 (FY130_21875)	1861396..1862304	+	909	WP_035208323.1	branched-chain amino acid ABC transporter permease	-
FY130_RS22025 (FY130_21880)	1862301..1863029	+	729	WP_262622540.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-70)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10611.03 Da
• Isoelectric Point: 8.0066

>T149627 WP_262622535.1 NZ_CP048585:1857581-1857862 [Agrobacterium pusense]
MIRSFKNKLTASIDDGSVKKGFPSDMLRRAQQLLTILDAANTVEDLRSPPGNRLEKLSGDREGQYSIRINKQWRICFVWT
EAGPESVEITDYH

Nucleotide

• Length: 282 bp

>T149627 NZ_CP064192:945404-945754 [Saccharothrix sp. 6-C]
ATGCGGCGAGGTGACATCTACTGGGTGGACCTGGAGCCGGCGCAGGGCGCCGAGGCCAACAAGATCCGCCCGGCCGTGGT
CGTCAGCAACGACGCGGCGAACCGGGCCGCCGGACGGGGTGGCGGCCGGGGTGTGGTGACGGTCGTGCCGATCACCTCCA
ACACCGCGAAGGTGTACCCGTTCCAGGTGCTGCTGCCCGCCAAGGACTGCGGGCTCGGCGCGGACTCGAAGGCCCAGGCC
GAGCAGGTGCGCACGGTCACCGCGGCCAGGCTCCGCGCCCGGATCGGCGGCCTGCCGCCGGCGGTGCTCAAGCAGCTCGA
CGAGGCACTGCGCGTGCACCTGTCGCTCTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11060.86 Da
• Isoelectric Point: 8.5148

>AT149627 WP_004444714.1 NZ_CP048585:1857918-1858217 [Agrobacterium pusense]
MTISKSPPIHPGEILRELYLEPLGMSAYALARHLNVPRTRIERIAGEKLGITADTALRLAKFFKTTPEYWLNLQNAFDIK
AAAARLETDLANIKELEAA

Nucleotide

• Length: 300 bp

>AT149627 NZ_CP064192:945187-945417 [Saccharothrix sp. 6-C]
ATGAAAATCAGTGTGAGCCTCCCCGAGGAGGACGTAGAGTTCGTTGATCGCTACCTGCGGCAACATCGGAGCGCATCGCG
GTCGTCGGTGATCCACCAGGCGATCACGCTGCTGCGCGAGGCGGGTCTGGAGAACGCCTACGCGAGCGCGTGGGAAGAGT
GGGAAGCCGGAGGAGACGCCGACATGTGGGACGTGACCGCGCCAGACGGTGTCCTCGATGCGGCGAGGTGA